CULTURE MEDIUM FOR GINGIVAL FIBROBLASTS

• US 20090061512A1
• Filed: 03/19/2008
• Published: 03/05/2009
• Est. Priority Date: 03/20/2007
• Status: Active Grant

• Current Assignee: Inserm Transfert SA (Allianz Global Investors GmbH), L'Assistance Publique - Hopitaux De Paris, Paris Descartes University, Universite Paris CITE
• Original Assignee: Université Paris Descartes
• Inventors: LAFONT, Antoine, COULOMB, Bernard, GOGLY, Bruno
• Granted Patent: US 7,951,593 B2
• Time in Patent Office: Days
• US Class Current: 435/366
• CPC Class Codes: C12N 2500/05 Inorganic components
• CPC Class Codes: C12N 2500/20 Transition metals
• CPC Class Codes: C12N 2500/25 Insulin-transferrin; Insulin-transferrin-selenium
• CPC Class Codes: C12N 2500/32 Amino acids
• CPC Class Codes: C12N 2500/84 from mammals
• CPC Class Codes: C12N 2500/90 Serum-free medium, which may still contain naturally-sourced components
• CPC Class Codes: C12N 2501/115 Basic fibroblast growth factor (bFGF, FGF-2)
• CPC Class Codes: C12N 5/0656 Adult fibroblasts

Abstract

The present invention relates to a gingival fibroblast culture medium free of animal serum, comprising an animal cell culture medium, free of animal serum, to which is added:

• • from 0.1 ng/ml to 100 ng/ml bFGF, and/or
  • from 1 μg/ml to 50 μg/ml insulin.

Claims

1. A gingival fibroblast culture medium free of animal serum, comprising a culture medium for animal or human cells, free of animal serum, to which is added:

from 0.1 ng/ml to 100 ng/ml bFGF, and/or

from 1 μ

g/ml to 50 μ

g/ml insulin.

2. The gingival fibroblast culture medium of claim 1, wherein bFGF is added at 1 ng/ml.

3. The gingival fibroblast culture medium of claim 1, wherein insulin is added at 5 μ

g/ml.

4. The gingival fibroblast culture medium according to claim 1, wherein the animal cell culture medium comprises 8 volumes DMEM and 2 volumes KNOCKOUT™

SR (INVITROGEN) or 9 volumes DMEM and 1 volume KNOCKOUT™

SR (INVITROGEN).

5. The gingival fibroblast culture medium according to claim 1, comprising bFGF at 1 ng/ml and insulin at 15 μ

g/ml.

6. The gingival fibroblast culture medium according to claim 1, further comprising platelet lysate.

7. The use of a gingival fibroblast culture as defined in claim 1, for the culture of gingival fibroblast.

8. A method for the culture of gingival fibroblasts which comprises growing cells in a gingival fibroblast culture as defined in claim 1.

9. A kit for cultivating gingival fibroblasts, comprising:

a gingival fibroblast culture medium as defined in claim 1, and

platelet lysate.