MASS DEFECT LABELING AND METHODS OF USE THEREOF

• US 20090061523A1
• Filed: 08/28/2007
• Published: 03/05/2009
• Est. Priority Date: 08/28/2006
• Status: Active Grant

• Current Assignee: The University of Georgia Research Foundation, Inc.
• Original Assignee: The University of Georgia Research Foundation, Inc.
• Inventors: Phillips, Robert S., Amster, I. Jonathan
• Granted Patent: US 7,951,602 B2
• Time in Patent Office: Days
• US Class Current: 436/86
• CPC Class Codes: C07K 1/13 Labelling of peptides
• CPC Class Codes: G01N 33/6848 Methods of protein analysis involving mass spectrometry
• CPC Class Codes: Y10T 436/24 Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Abstract

Briefly described, embodiments of this disclosure include mass defect labeled peptides, methods of identifying peptides, and the like.

Claims

1. A peptide comprising:

a mass defect labeled peptide including at least one cysteine, wherein at least one of the cysteine residues are labeled with 2,4-dibromo-acetanilide.

2. The peptide of claim 1, wherein the mass defect labeled peptide includes at least one tryptophan, wherein at least one of the tryptophan residues are labeled with 4,6-dibromo-2-trifluoromethylphenylsulfenyl chloride

3. A method of identifying peptides comprising:

labeling at least one cysteine in a target peptide with a mass defect label, wherein the mass defect label is 2,4-dibromo-acetanilide;

introducing the target peptide to a mass spectrometry system; and

obtaining a mass spectrum of the target peptide.

4. The method of claim 3, further comprising:

labeling at least one tryptophan in the target peptide with a mass defect label, wherein the mass defect label is 4,6-dibromo-2-trifluoromethylphenylsulfenyl chloride.

5. The method of claim 3, wherein the mass spectrometry system is selected from an ion trap mass analyzer system (IT-MS), an ion cyclotron resonance mass analyzer system (ICR-MS), and an orbitrap system.

6. A peptide comprising:

a mass defect labeled peptide including at least one tryptophan, wherein at least one of the tryptophan residues are labeled with 4,6-dibromo-2-trifluoromethylphenylsulfenyl chloride.

7. A method of identifying peptides comprising:

labeling at least one tryptophan in a target peptide with a mass defect label, wherein the mass defect label is 4,6-dibromo-2-trifluoromethylphenylsulfenyl chloride;

introducing the target peptide to a mass spectrometry system; and

obtaining a mass spectrum of the target peptide.

8. The method of claim 7, wherein the mass spectrometry system is selected from an ion trap mass analyzer system (IT-MS), an ion cyclotron resonance mass analyzer system (ICR-MS), and an orbitrap system.