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NUCLEIC ACID SEQUENCING BY SELECTIVE EXCITATION OF MICROPARTICLES

  • US 20090061526A1
  • Filed: 08/28/2007
  • Published: 03/05/2009
  • Est. Priority Date: 08/28/2007
  • Status: Active Grant
First Claim
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1. A method for sequencing nucleic acid microparticles, the method comprising the steps of:

  • performing at least a sequencing reaction on the nucleic acid microparticles using one or more sequencing reagents;

    selectively exciting the nucleic acid microparticles in an excitation pattern;

    optically imaging the nucleic acid microparticles at a resolution insufficient to resolve individual microparticles; and

    processing the optical images of the nucleic acid microparticles using information on the excitation pattern to determine presence or absence of at least an optical signature, the presence or absence of said at least optical signature indicating sequence information of the nucleic acid.

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