DYNAMIC MODULATION FOR MULTIPLEXATION OF MICROFLUIDIC AND NANOFLUIDIC BASED BIOSENSORS
First Claim
1. A multichannel particle counting device comprising:
- a plurality of microfluidic channels for dividing a first reservoir and a second reservoir, and for maintaining fluid communication between the first and second reservoirs;
each microfluidic channel including a control electrode, wherein each control electrode is substantially electrically isolated from any other control electrode;
each control electrode encoded to respond to a specific frequency;
the first and second reservoirs together including one set of detection electronics including a first electrode in electrical communication with a power supply and a second electrode in electrical communication with a measuring circuit;
the one set of detection electronics being the collector for each signal from each control electrode and having a means to deconvolute the collected signals;
the reservoirs containing an electrolyte solution containing particles to be counted; and
a means for creating a net fluid flow of electrolyte from one reservoir to the other reservoir through the microfluidic channels.
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Accused Products
Abstract
The present invention generally relates to a method for rapidly counting micron and/or submicron particles by passing such particles through any of a plurality of microfluidic channels simultaneously with an ion current and measuring the signal generated thereby. The present invention also generally relates to a device for practicing the method of the present invention. Some embodiments can include methods and/or devices for distinguishing between and counting particles in mixtures. Still other embodiments can include methods and/or devices for identifying and/or counting bioparticles and/or bioactive particles such as pollen.
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Citations
23 Claims
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1. A multichannel particle counting device comprising:
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a plurality of microfluidic channels for dividing a first reservoir and a second reservoir, and for maintaining fluid communication between the first and second reservoirs; each microfluidic channel including a control electrode, wherein each control electrode is substantially electrically isolated from any other control electrode; each control electrode encoded to respond to a specific frequency; the first and second reservoirs together including one set of detection electronics including a first electrode in electrical communication with a power supply and a second electrode in electrical communication with a measuring circuit; the one set of detection electronics being the collector for each signal from each control electrode and having a means to deconvolute the collected signals; the reservoirs containing an electrolyte solution containing particles to be counted; and a means for creating a net fluid flow of electrolyte from one reservoir to the other reservoir through the microfluidic channels. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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Specification