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Use of Copper Glutamate in Cell Culture for Production of Polypeptides

  • US 20090068705A1
  • Filed: 02/29/2008
  • Published: 03/12/2009
  • Est. Priority Date: 03/02/2007
  • Status: Active Grant
First Claim
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1. A method of producing a polypeptide in a cell culture comprising steps of:

  • culturing mammalian cells that contain a gene encoding a polypeptide of interest in a cell culture medium comprising between approximately 0.5 and 5 μ

    M copper and between approximately 1.7 and 33 mM glutamate under conditions and for a time sufficient to permit expression of the polypeptide, wherein the fraction of misfolded and/or aggregated polypeptide, relative to the total polypeptide produced, is decreased compared to the fraction of misfolded and/or aggregated polypeptide that would be observed under otherwise identical conditions in an otherwise identical medium that lacks copper and glutamate.

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