Cell culture scaffold, methods of manufacturing the scaffold and cell culture methods

• US 20090068739A1
• Filed: 08/29/2008
• Published: 03/12/2009
• Est. Priority Date: 06/30/2004
• Status: Active Grant

• Current Assignee: National University Corporation Hokkaido University
• Original Assignee: National University Corporation Hokkaido University
• Inventors: Chen, Yongmei, Osada, Yoshihito, Gong, Jian Ping
• Granted Patent: US 8,158,425 B2
• Time in Patent Office: Days
• US Class Current: 435/397
• CPC Class Codes: C12M 25/14 Scaffolds; Matrices in general C12N5/0068
• CPC Class Codes: C12N 2533/30 Synthetic polymers
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06

Abstract

A cell culture scaffold containing a gel having a network structure comprised of a synthetic polymer such that cultured cells spread in a shorter time and the number of adsorbed cultured cells per unit area is larger than in the case of using a gel having a network structure comprised of polyacrylic acid, while taking advantage of synthetic polymers with low manufacturing cost, easy quality control and no risk of virus infection in cultured cell. Used as the cell culture scaffold is a gel containing a synthetic polymer obtained by polymerization or copolymerization of a monomer having a sulfonic group such as p-styrenesulfonic acid sodium salt (NaSS) and 2-acrylamide-2-methylpropane sulfonic acid sodium salt (NaAMPS).

Claims

1. A scaffold for culturing cells containing a gel comprising:

an interpenetrating polymer network structure in which a network structure comprising a synthetic polymer obtained by polymerization or copolymerization of a p-styrenesulfonic acid alkali metal salt or a 2-acrylamide-2-methylpropane sulfonic acid alkali metal salt penetrates a network structure comprising a polymer having a crosslinking structure, or

a semi-interpenetrating polymer network structure in which the synthetic polymer is a linear polymer and the linear synthetic polymer penetrates the network structure with the crosslinking structure.

2. The scaffold for culturing cells according to claim 1, wherein the network structure comprising the polymer having the crosslinking structure is bacteria cellulose.

3. The scaffold for culturing cells according to claim 1, wherein the gel comprises the interpenetrating polymer network structure.

4. A method for culturing cells including the step of culturing cells on the scaffold according to claim 1.