AMORPHOUS CELL DELIVERY VEHICLE TREATED WITH PHYSICAL/PHYSICOCHEMICAL STIMULI
First Claim
1. A composition comprising a cell/matrix composition produced by contacting a population of cells selected for in vitro culture with a biodegradable amorphous carrier;
- placing the contacted population of cells in a cell space for receiving the cells, said cell space being bounded at least in part by a semi-permeable membrane having a molecular weight cut-off greater than 100 kDa and up to 1,000 kDa; and
periodically applying pressure to the contacted population of cells.
1 Assignment
0 Petitions
Accused Products
Abstract
Provided are compositions and methods for in vitro generation and in vivo use of tissue for the repair of defective tissue, especially cartilage. Chondrocytes or other cells are cultured in vitro in a biodegradable amorphous carrier within the confines of a space bounded by a semi-permeable membrane with a molecular weight cut-off of greater than 100 kDa. The culture can be subjected to physical/physicochemical conditions that mimic in vivo conditions of the tissue in need of repair or replacement. In one embodiment the invention provides an amorphous preparation of chondrocytes and their extracellular products, suitable for injection.
-
Citations
11 Claims
-
1. A composition comprising a cell/matrix composition produced by contacting a population of cells selected for in vitro culture with a biodegradable amorphous carrier;
- placing the contacted population of cells in a cell space for receiving the cells, said cell space being bounded at least in part by a semi-permeable membrane having a molecular weight cut-off greater than 100 kDa and up to 1,000 kDa; and
periodically applying pressure to the contacted population of cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
- placing the contacted population of cells in a cell space for receiving the cells, said cell space being bounded at least in part by a semi-permeable membrane having a molecular weight cut-off greater than 100 kDa and up to 1,000 kDa; and
Specification