Rapid Comparative Genome Hybridization
First Claim
Patent Images
1. A method for performing nucleic acid hybridization to immobilized nucleic acid, comprising the following steps:
- a) contacting a solid support comprising one or more immobilized nucleic acid probe molecules under hybridization conditions with a non-specific blocking nucleic acid at a concentration of at least about 1 mg/ml, said one or more immobilized nucleic acid probe molecules capable of hybridizing with a sequence complementary thereto;
b) contacting the solid support under hybridization conditions with a test sample containing nucleic acid target molecules;
c) applying acoustic surface waves to the hybridization of step a) or stepb) or both; and
d) determining whether the one or more nucleic acid probes of the solid support have hybridized to test sample nucleic acid target molecules.
0 Assignments
0 Petitions
Accused Products
Abstract
Disclosed is a method for performing nucleic acid hybridization assays, such as assays used for detecting and mapping chromosomal or genetic abnormalities associated with various diseases or associated with predisposition to various diseases. In a particular aspect, the present method relates to the use of rapid nucleic acid hybridization methods for comparing nucleic acid segments of one genome to corresponding nucleic acid segments in another genome(s).
-
Citations
38 Claims
-
1. A method for performing nucleic acid hybridization to immobilized nucleic acid, comprising the following steps:
-
a) contacting a solid support comprising one or more immobilized nucleic acid probe molecules under hybridization conditions with a non-specific blocking nucleic acid at a concentration of at least about 1 mg/ml, said one or more immobilized nucleic acid probe molecules capable of hybridizing with a sequence complementary thereto; b) contacting the solid support under hybridization conditions with a test sample containing nucleic acid target molecules; c) applying acoustic surface waves to the hybridization of step a) or step b) or both; and d) determining whether the one or more nucleic acid probes of the solid support have hybridized to test sample nucleic acid target molecules. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
-
-
18. A method for performing nucleic acid hybridization to immobilized nucleic acid, comprising the following steps:
-
a) contacting a solid support containing one or more immobilized nucleic acid probe molecules under hybridization conditions with a nucleic acid test sample containing one or more nucleic acid target molecules, said one or more immobilized nucleic acid probe molecules capable of hybridizing with a sequence complementary thereto; b) applying acoustic surface waves to the hybridization of step a); and c) determining whether the one or more nucleic acid probes of the solid support have hybridized to test sample nucleic acid target molecules. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 33, 34, 35, 36, 37, 38)
-
-
32. The method of claim 32 wherein said reference sample and said test sample are labeled.
Specification