Biomolecular Attachment Sites on Microelectronic Arrays and Methods Thereof
First Claim
1. A method of addressing a biomolecule to a selectively addressable electrode, comprising the steps of:
- providing a permeation layer overlying a plurality of selectively addressable electrodes, the permeation layer comprising a reactive group adapted to bond to a biomolecule, wherein the reactive group requires activation through a chemical transformation before bonding to the biomolecule;
biasing at least one selectively addressable electrode such that a pH change occurs in an overlying solution of the at least one selectively addressable electrode, wherein the reactive group in a portion of the permeation layer above the at least one selectively addressable electrode is chemically transformed to an activated reactive group as a result of the pH change; and
binding a biomolecule to the permeation layer overlying the at least one selectively addressable electrode through the activated reactive group.
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Abstract
Methods of addressing a biomolecule to a selectively addressable electrode are described. A permeation layer overlying a plurality of selectively addressable electrodes is provided. The permeation layer includes a reactive group that is adapted to bond to a biomolecule and that requires activation through a chemical transformation before bonding to the biomolecule. At least one selectively addressable electrode is biased such that a pH change occurs in an overlying solution of the at least one selectively addressable electrode. The reactive group in a portion of the permeation layer above the at least one selectively addressable electrode is then chemically transformed to an activated reactive group as a result of the pH change. A biomolecule is then bound to the permeation layer overlying the at least one selectively addressable electrode through the activated reactive group.
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Citations
8 Claims
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1. A method of addressing a biomolecule to a selectively addressable electrode, comprising the steps of:
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providing a permeation layer overlying a plurality of selectively addressable electrodes, the permeation layer comprising a reactive group adapted to bond to a biomolecule, wherein the reactive group requires activation through a chemical transformation before bonding to the biomolecule; biasing at least one selectively addressable electrode such that a pH change occurs in an overlying solution of the at least one selectively addressable electrode, wherein the reactive group in a portion of the permeation layer above the at least one selectively addressable electrode is chemically transformed to an activated reactive group as a result of the pH change; and binding a biomolecule to the permeation layer overlying the at least one selectively addressable electrode through the activated reactive group. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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Specification