Continuous monitor sensor with covalently bound enzyme
First Claim
1. An electrochemical sensor for determining an analyte in a fluid medium, comprising:
- at least one reference electrode;
at least one working electrode comprising,particles of an electrocatalyst in an electrode matrix, andan enzyme that is suitable for determining an analyte is selectively covalently bound to the particles of the electrocatalyst.
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Abstract
The disclosure concerns a high efficiency electrochemical sensor with high signal yield for determining an analyte in a fluid medium comprising, at least one reference electrode, at least one working electrode having particles of an electrocatalyst in an electrode matrix, and an enzyme that is suitable for determining an analyte is selectively covalently bound to the particles of the electrocatalyst. The disclosure also describes a process for producing the electrochemical sensor and a method for determining an analyte in a fluid medium using the electrochemical sensor. have a high efficiency and thus achieve a high signal yield.
24 Citations
35 Claims
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1. An electrochemical sensor for determining an analyte in a fluid medium, comprising:
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at least one reference electrode; at least one working electrode comprising, particles of an electrocatalyst in an electrode matrix, and an enzyme that is suitable for determining an analyte is selectively covalently bound to the particles of the electrocatalyst. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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33. A method of manufacture for producing an electrochemical sensor, comprising the steps of:
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(a) providing electrocatalyst particles; (b) coating the electrocatalyst particles with an enzyme, wherein the enzyme is covalently bound to the electrocatalyst particles; (c) mixing the electrocatalyst particles coated covalently with enzyme that are obtained in step (b) with a conductive electrode material; (d) processing the mixture obtained in step (c) to form an electrode, and (e) combining the electrode obtained in step (d) with at least one further electrode. - View Dependent Claims (34)
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35. A method for determining an analyte in a fluid medium, comprising the steps of:
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(a) contacting the fluid medium with a reference electrode; (b) contacting the fluid medium with a working electrode comprising;
particles of an electrocatalyst in an electrode matrix, and an enzyme that is suitable for determining an analyte is selectively covalently bound to the particles of the electrocatalyst.(c) determining the presence or/and the amount of analyte in the fluid medium by measuring a signal generated by the electrochemical sensor.
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Specification