Systems and methods for volumetric tissue scanning microscopy
First Claim
1. A multifocal imaging system comprising:
- a multiphoton light source;
a multifocal optical device that provides a plurality of optical pathways;
a scanner that provides relative movement between the plurality of optical pathways and a material to be imaged;
an optical system that couples light from the optical device onto a region of interest of the material;
a detector array that detects light from a plurality of focal locations in the region of interest to generate image data, the detector array having a plurality of detector elements correlated with the focal locations; and
an image processor connected to the detector array that generates three dimensional image data of the material.
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Accused Products
Abstract
In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.
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Citations
50 Claims
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1. A multifocal imaging system comprising:
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a multiphoton light source; a multifocal optical device that provides a plurality of optical pathways; a scanner that provides relative movement between the plurality of optical pathways and a material to be imaged; an optical system that couples light from the optical device onto a region of interest of the material; a detector array that detects light from a plurality of focal locations in the region of interest to generate image data, the detector array having a plurality of detector elements correlated with the focal locations; and an image processor connected to the detector array that generates three dimensional image data of the material. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. A method for multifocal imaging comprising:
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illuminating a region of interest with light having at least two photons using a plurality of optical pathways; providing relative movement between the plurality of optical pathways and the region of interest to scan a plurality of focal locations in three dimensions; and detecting light from a plurality of focal locations in the region of interest to generate three dimensional image data. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36)
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37. A system for providing a three-dimensional image of a region of interest, comprising:
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a light source for producing excitation light and providing at least two photons onto the region of interest; a scanning microscope optically coupled to the light source; a tissue sectioning device coupled to the microscope; an x-y scanner to scan the region of interest; an image sensor that detects a plurality of images of the region of interest; and a data processor that processes the plurality of images to produce a processed three-dimensional image of the region of interest. - View Dependent Claims (38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50)
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Specification