APPARATUS AND METHOD FOR RAMAN SPECTROSCOPY AND MICROSCOPY WITH TIME DOMAIN SPECTRAL ANALYSIS
First Claim
1. A method of performing stimulated Raman spectroscopy of a sample, comprising:
- exposing a sample to beams of pulsed probe light;
exposing said sample to at least one beam of a second light during said exposure of said probe light;
mapping a spectrum of light scattered from said sample into a time-domain waveform with a plurality of dispersive elements so that the spectra can be detected directly in the time domain; and
detecting said time-domain waveform.
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Abstract
An apparatus and method for measuring Raman-type spectra using optical dispersion to convert an optical spectrum into a waveform which can be detected directly in the time domain without the use of a conventional spectrometer. In the example of stimulated Raman spectroscopy, the apparatus and method exposes a sample to a chirped, pulsed probe beam and a Raman pump beam and the resulting Raman spectra is detected by an optical detector in the time domain, and analyzed. Alternatively, the Raman spectra from the probe and pump beams is chirped with a dispersive element prior to detection and analysis. Each probe pulse provides a snapshot of the Raman spectrum that is sampled in time so that neither repetitive waveforms nor static samples are required. Therefore, high speed acquisitions and high throughput assays can be conducted. To facilitate detection, these spectral signals can also be amplified using distributed Raman amplification directly in the dispersive element.
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Citations
54 Claims
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1. A method of performing stimulated Raman spectroscopy of a sample, comprising:
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exposing a sample to beams of pulsed probe light; exposing said sample to at least one beam of a second light during said exposure of said probe light; mapping a spectrum of light scattered from said sample into a time-domain waveform with a plurality of dispersive elements so that the spectra can be detected directly in the time domain; and detecting said time-domain waveform. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method for conducting Raman microscopy of a sample, comprising:
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placing a sample in proximity to a metal probe; exposing said sample to beams of chirped, pulsed laser light; exposing said sample to at least one beam of light during said exposure of chirped, pulsed laser light; and analyzing characteristics of light scattered from said sample during exposure to said beams of said light. - View Dependent Claims (17, 18, 19, 20)
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21. A method of spontaneous Raman spectroscopy, comprising:
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exposing a sample to a beam of pulsed light; dispersing temporally light emitted from said sample; mapping a spectrum of light scattered from said sample into a time domain waveform; and characterizing said time domain waveform. - View Dependent Claims (22, 23, 24)
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25. An apparatus for Raman spectroscopy of a sample, comprising:
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a pulsed probe laser; a dispersive element operably coupled to said pulsed laser configured to direct temporally dispersed pulsed laser light on a sample; a Raman pump laser configured to direct laser light on said sample; and an optical detector; wherein characteristics of light scattered from said sample during exposure to said laser light can be analyzed directly in a time domain. - View Dependent Claims (26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39)
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40. An apparatus for Raman spectroscopy, comprising:
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a pulsed probe laser; a first dispersive element operably coupled to said pulsed probe laser configured to direct temporally dispersed pulsed laser light on a sample; a Raman pump laser configured to direct laser light on said sample; a second dispersive element adapted to receive and disperse light scattered from said sample during exposure to said pulsed laser light and said Raman pump laser light; and means for analyzing characteristics of light scattered from said sample sampled within a time domain. - View Dependent Claims (41, 42, 43)
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44. An apparatus for Raman Microscopy, comprising:
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a source of a plurality of chirped, pulsed probe beams directed to a sample; a source of a plurality of Raman pump beams directed to a sample; and means for detecting characteristics of light scattered from exposure of a sample to at least one of said probe beams and at least one of said pump beams within a time domain. - View Dependent Claims (45, 46, 47, 48, 49, 50, 51, 52, 53, 54)
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Specification