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Non-hemolytic LLO fusion proteins and methods of utilizing same

  • US 20090081248A1
  • Filed: 06/23/2008
  • Published: 03/26/2009
  • Est. Priority Date: 03/26/2001
  • Status: Active Grant
First Claim
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1. A recombinant protein comprising a listeriolysin O (LLO) protein or N-terminal fragment thereof, wherein said LLO protein or N-terminal fragment comprises a mutation in a cholesterol-binding domain (CBD), wherein said mutation comprises:

  • a. a substitution of 1-50 μ

    amino acid peptide in said CBD as set forth in SEQ ID NO;

    18 for a 1-50 amino acid non-LLO peptide;

    b. a mutation of residue C484, W491, or W492 of SEQ ID NO;

    37 or a combination thereof;

    orc. a deletion of a 1-50 amino acid peptide in said CBD as set for in SEQ ID NO;

    18.wherein said recombinant protein exhibits a greater than 100-fold reduction in hemolytic activity relative to wild-type LLO.

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