METHOD OF ASSUMING DRUG SENSITIVITY TO CDK4 INHIBITOR

• US 20090081645A1
• Filed: 08/02/2005
• Published: 03/26/2009
• Est. Priority Date: 08/02/2004
• Status: Abandoned Application

• Current Assignee: MSD Kabushiki Kaisha (Merck & Co., Inc.)
• Original Assignee: MSD Kabushiki Kaisha (Merck & Co., Inc.)
• Inventors: Eguchi, Tomohiro, Yamanaka, Kazunori, Itadani, Hiraku, Kotani, Hidehito, Shimomura, Toshiyasu
• Application Number:
  US11/659,258
• Publication Number:
  US 20090081645A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: C12Q 1/485 involving kinase

Abstract

It is intended to provide a gene for assuming drug sensitivity, which is to be used for assuming the drug sensitivity to a CDK4 inhibitor based on the expression amount in a test tissue or a test cell, selected from the group consisting of p16 gene, p18 gene and p27 gene. It is also intended to provide a method of assuming the drug sensitivity to a CDK4 inhibitor by using the expression amount of the above gene in a test tissue or a test cell as an indication.

Claims

1. A gene for assuming drug sensitivity, which is to be used for assuming the drug sensitivity to a CDK4 inhibitor based on the expression amount in a test tissue or a test cell, selected from the group consisting of p16 gene, p18 gene and p27 gene.

2. The gene for assuming drug sensitivity according to claim 1, wherein the gene selected from the group consisting of p16 gene, p18 gene and p27 gene is p18 gene.

3. The gene for assuming drug sensitivity according to claim 1, wherein the drug sensitivity is drug resistance.

6. An antibody for detection of drug sensitivity to a CDK4 inhibitor which specifically recognizes a protein expressed by a gene of claim 1.

4. A reagent for detection of drug sensitivity to a CDK4 inhibitor containing an antibody which specifically recognizes an antisense nucleic acid of gene selected from the group consisting of p16 gene, p18 gene and p27 gene or protein expressed by said gene.

5. An antisense nucleic acid of a gene selected from the group consisting of p16 gene, p18 gene and p27 gene.

7. A method of measuring the drug sensitivity to a CDK4 inhibitor comprising the steps of:

measuring the expression amount of at least one gene selected from the group consisting of p16 gene, p18 gene and p27 gene in a test tissue or in a test cell and

comparing the expression amount of said gene with the expression amount of said gene in a healthy tissue or in a healthy cell.

8. The method of assuming according to claim 7, wherein a step where it is judged that drug sensitivity of a test tissue or a test cell to a CDK4 inhibitor is high is further included therein in case expression of the above gene in a test tissue or in a test cell is lower than expression of the above gene in a healthy tissue or in a healthy cell as a result of comparison of the expression amounts.

11. The method according to claim 7, wherein the gene is p18 gene.

9. A method of assuming drug sensitivity to a CDK4 inhibitor including a step where expression of at least one gene selected from the group consisting of p16 gene, p18 gene and p27 gene in a test tissue or a test cell is detected.

10. A method of assuming according to claim 9, wherein a step where drug sensitivity of the above-mentioned test tissue or test cell to a CDK4 inhibitor is judged to be low is further included in case the above-mentioned gene is expressed as a result of the detection.

12. Use of a gene selected from the group consisting of p16 gene, p18 gene and p27 gene for assuming drug sensitivity to a CDK4 inhibitor based on the expression amount in a test tissue or a test cell.