METHOD FOR FORMULATING AND IMMOBILIZING A MATRIX PROTEIN AND A MATRIX PROTEIN FOR USE IN A SENSOR
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Abstract
A method for formulating and immobilizing a protein and a protein matrix formed by the method. The protein matrix preparation method results in a physically and chemically stable protein matrix that has low swelling, non-leaching, high activity, and high mechanical strength properties. The method includes cross-linking and hardening the protein mixture and using a mold to form a protein into a desired shape and size.
66 Citations
49 Claims
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1-31. -31. (canceled)
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32. A method of forming a molded protein matrix comprising the steps of:
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combining a protein mixture with a cross-linking reagent; placing the combined protein mixture and cross-linking reagent into a mold, where the mold comprises an internal volume with three-dimensional features; and incubating the combined protein mixture and cross-linking reagent in the internal volume of the mold to form a molded protein matrix having a three-dimensional shape that corresponds to three-dimensional features of the internal volume of the mold. - View Dependent Claims (33, 34, 35, 36, 37, 38)
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- 39. A molded protein matrix formed by contacting a protein mixture with a cross-linking reagent in three separate steps, wherein at least one step of cross-linking occurs in a mold.
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41. A method of forming a molded protein matrix comprising:
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containing a protein mixture and a first cross-linking reagent in a mold comprising an internal volume with three-dimensional features and incubating the combined protein mixture and first cross-linking reagent in the internal volume of the mold to form a molded protein matrix having a three-dimensional shape that corresponds to three-dimensional features of the internal volume of the mold; and exposing the molded protein matrix to a second cross-linking reagent to further cross link the molded protein mixture. - View Dependent Claims (42, 43, 44, 45, 46, 47, 48, 49)
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Specification