METHOD FOR FORMULATING AND IMMOBILIZING A MATRIX PROTEIN AND A MATRIX PROTEIN FOR USE IN A SENSOR

• US 20090081753A1
• Filed: 07/24/2008
• Published: 03/26/2009
• Est. Priority Date: 10/23/2001
• Status: Active Grant

• Current Assignee: Medtronic Minimed Incorporated (Medtronic Plc)
• Original Assignee: Medtronic Minimed Incorporated (Medtronic Plc)
• Inventors: Reghabi, Bahar, Shah, Rajiv, Montalvo, Rudolph A., Zhang, Yanan
• Granted Patent: US 8,187,851 B2
• Time in Patent Office: Days
• US Class Current: 435/177
• CPC Class Codes: C07K 1/1077 by covalent attachment of residues other than amino acids or peptide residues, e.g. sugars, polyols, fatty acids
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A method for formulating and immobilizing a protein and a protein matrix formed by the method. The protein matrix preparation method results in a physically and chemically stable protein matrix that has low swelling, non-leaching, high activity, and high mechanical strength properties. The method includes cross-linking and hardening the protein mixture and using a mold to form a protein into a desired shape and size.

Claims

1-31. -31. (canceled)

32. A method of forming a molded protein matrix comprising the steps of:

combining a protein mixture with a cross-linking reagent;

placing the combined protein mixture and cross-linking reagent into a mold, where the mold comprises an internal volume with three-dimensional features; and

incubating the combined protein mixture and cross-linking reagent in the internal volume of the mold to form a molded protein matrix having a three-dimensional shape that corresponds to three-dimensional features of the internal volume of the mold.

33. The method of claim 32, further comprising an additional cross-linking step comprising exposing the molded protein matrix to another cross-linking reagent.

34. The method of claim 33, wherein the primary shape of the protein matrix is imparted during the first cross-linking process.

35. The method of claim 32, wherein the three-dimensional features of the internal volume of the mold define a generally semi-cylindrical cross-section such that the molded protein matrix has at least a portion of a cross-section having a generally semi-cylindrical shape.

36. The method of claim 32, wherein the three-dimensional features of the internal volume of the mold define at least one of a generally rectangular, generally cylindrical, generally tubular or generally spherical cross-section such that the molded protein matrix has at least a portion of a cross-section having at least one of a generally rectangular, generally cylindrical, generally tubular or generally spherical shape.

37. A molded protein matrix formed according to the method of claim 32.

38. A sensor device comprising a molded protein matrix according to the method of claim 32, located in a sensor device housing, wherein the sensor device is adapted to contact at least one biological fluid.

39. A molded protein matrix formed by contacting a protein mixture with a cross-linking reagent in three separate steps, wherein at least one step of cross-linking occurs in a mold.

40. A sensor device comprising:

a housing; and

a molded protein matrix according to claim 39 located inside the housing,

wherein the sensor device is adapted to contact at least one biological fluid.

41. A method of forming a molded protein matrix comprising:

containing a protein mixture and a first cross-linking reagent in a mold comprising an internal volume with three-dimensional features and incubating the combined protein mixture and first cross-linking reagent in the internal volume of the mold to form a molded protein matrix having a three-dimensional shape that corresponds to three-dimensional features of the internal volume of the mold; and

exposing the molded protein matrix to a second cross-linking reagent to further cross link the molded protein mixture.

42. The method of claim 41, wherein exposing the protein matrix to a second cross-linking reagent occurs while the molded protein is in a mold.

43. The method of claim 42, wherein the mold in which the protein matrix is exposed to a second cross-linking reagent is the same mold in which the protein mixture and the first cross-lining reagent are contained for incubating.

44. The method of claim 41, wherein the three-dimensional features of the internal volume of the mold define a generally semi-cylindrical cross-section such that the molded protein matrix has at least a portion of a cross-section having a generally semi-cylindrical shape.

45. The method of claim 41, wherein the three-dimensional features of the internal volume of the mold define at least one of a generally rectangular, generally cylindrical, generally tubular or generally spherical cross-section such that the molded protein matrix has at least a portion of a cross-section having at least one of a generally rectangular, generally cylindrical, generally tubular or generally spherical shape.

46. A molded protein matrix formed according to the method of claim 41.

47. A sensor device comprising:

a housing; and

a molded protein matrix according to claim 41 located inside the housing wherein the sensor device is adapted to contact at least one biological fluid that causes a detectable reaction in the molded protein matrix.

48. The method of claim 47, wherein the three-dimensional features of the internal volume of the mold define a generally semi-cylindrical cross-section such that the molded protein matrix has at least a portion of a cross-section having a generally semi-cylindrical shape.

49. The method of claim 47, wherein the three-dimensional features of the internal volume of the mold define at least one of a generally rectangular, generally cylindrical, generally tubular or generally spherical cross-section such that the molded protein matrix has at least a portion of a cross-section having at least one of a generally rectangular, generally cylindrical, generally tubular or generally spherical shape.