REACTIVE SURFACE ON A POLYMERIC SUBSTRATE
First Claim
Patent Images
1. A method for making a polymer substrate having a working surface upon which biologically active compounds can bind comprising:
- providing a cyclic polyolefin copolymer;
treating the cyclic polyolefin copolymer with plasma to provide functional groups on a surface of the polymer substrate;
exposing the plasma-treated copolymer to a conjugate so that the functional groups on the surface of the polymer substrate form covalent bonds with the conjugate;
exposing the polymer surface covalently bound to the conjugate to a biologically active compound so that the biologically active compound is immobilized on the polymer surface.
1 Assignment
0 Petitions
Accused Products
Abstract
Plasma treated cyclic polyolefin copolymer surfaces having enhanced binding density for binding biologically active agents and cells are provided. These plasma treated cyclic polyolefin copolymer surfaces may be further enhanced for binding biologically active agents or cells by the application of conjugates. Methods of making and characterizing treated polymer surfaces are also provided.
-
Citations
21 Claims
-
1. A method for making a polymer substrate having a working surface upon which biologically active compounds can bind comprising:
-
providing a cyclic polyolefin copolymer; treating the cyclic polyolefin copolymer with plasma to provide functional groups on a surface of the polymer substrate; exposing the plasma-treated copolymer to a conjugate so that the functional groups on the surface of the polymer substrate form covalent bonds with the conjugate; exposing the polymer surface covalently bound to the conjugate to a biologically active compound so that the biologically active compound is immobilized on the polymer surface. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
-
-
10. A method for making a polymer substrate having a working surface upon which cells can bind comprising:
-
providing a cyclic polyolefin copolymer; treating the cyclic polyolefin copolymer with plasma to provide functional groups on a surface of the polymer substrate; exposing the polymer surface to a conjugate so that the functional groups on the surface of the polymer substrate form covalent bonds with the conjugate; exposing the polymer surface with covalently bound conjugate to a cell-binding peptide so that the cell binding peptide becomes immobilized on the polymer surface. - View Dependent Claims (11, 12, 13, 14, 15, 16)
-
-
17. A method for assessing the number of accessible functional groups on the surface of a polymer available for binding comprising;
-
providing a plasma-treated cyclic polyolefin copolymer surface having a number of functional groups on its surface to be determined; exposing the plasma-treated cyclic polyolefin copolymer surface to 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS); exposing the plasma-treated cyclic polyolefin copolymer surface to labeled biological agent or a mixture or labeled and unlabeled biological agents so that biological agent is immobilized to the surface, and; determining the amount of label indirectly bound to the surface. - View Dependent Claims (18)
-
- 19. A polymer substrate having a working surface upon which cells can bind comprising a cyclic polyolefin copolymer substrate having plasma-treatment-induced functional groups conjugated to cell-binding peptides.
-
21. A method for assigning a rating to a surface comprising:
-
providing a treated polymer surface having a number of functional groups on its surface to be determined; exposing the polymer surface to conjugate so that the functional groups on the surface of the polymer form covalent bonds with the conjugate; exposing the polymer surface covalently bound to the conjugate to labeled biological agent or a mixture or labeled and unlabeled biological agents so that the labeled biological agent becomes immobilized to the polymer surface; determining the amount of label immobilized to the polymer surface; assigning a rating to the polymer surface based on the amount of label immobilized to the surface.
-
Specification