CONJUGATE PROBES AND OPTICAL DETECTION OF ANALYTES

• US 20090082214A1
• Filed: 12/21/2007
• Published: 03/26/2009
• Est. Priority Date: 03/09/2001
• Status: Abandoned Application

• Current Assignee: Trovagene, Inc.
• Original Assignee: Apollo Technologies Incorporated (Ecolab, Inc.)
• Inventors: Liu, Zhiping, Li, Zheng
• Application Number:
  US11/963,725
• Publication Number:
  US 20090082214A1
• Time in Patent Office: Days
• US Class Current: 506/9
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: B01J 2219/00646 the compounds being bound to beads immobilised on the solid supports
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00704 integrated with the reactor apparatus
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/00729 Peptide nucleic acids [PNA]
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/548 Carbohydrates, e.g. dextran

Abstract

This invention relates to conjugate probes and optical detection of analytes. More specifically, this invention relates to conjugate probes which are used to form an array for a biosensor employing optical detection of analytes.

Claims

1. A conjugate for detecting analytes with a sensor comprising a chemical or biomolecule coupled to a 1,2-diol-containing polymer.

19. An array for detecting analytes with an image sensor comprising a conjugate as in claim 1.

22. An array as in claim 19 wherein the image sensor comprises a CMOS Image sensor.

23. A method of detecting analytes comprising contacting a fluid containing target analytes with an array as in claim 19.

2. A conjugate for detecting analytes with a sensor comprising a chemical or biomolecule coupled to a linear polysaccharide.

3. A conjugate for detecting analytes with a sensor comprising a chemical or biomolecule coupled to a branched polysaccharide.

4. A conjugate for detecting analytes with a sensor comprising a chemical or biomolecule coupled to a polynucleotide having a hydroxyl group vicinal to a phosphodiester linkage.

5. A conjugate for detecting analytes with a sensor comprising a chemical or biomolecule coupled to a polymer selected from the group consisting of RNA, poly(T), poly(U), and poly(A).

6. A conjugate comprising a chemical or biomolecule coupled to a branched polysaccharide selected from the group consisting of amylose, amylopectin, glycogen, dextran, cellulose, chitin, chitosan, peptidoglycan, glycosaminoglycan, and mixtures thereof.

7-11. -11. (canceled)

12. A method of making a conjugate comprising,

reacting a polysaccharide with an oxidizing agent, thereby forming a polysaccharide having aldehyde groups;

and

adding a reactive amino group to each of a plurality of chemical or biomolecules; and

coupling a plurality of the aldehyde groups to the plurality of chemical or biomolecules.

13. The method of claim 12 wherein the oxidizing agent is sodium periodate.

14. The method of claim 12 wherein the coupling of the plurality of aldehyde groups to at least one of the plurality of chemical or biomolecules is done with a photoreactive crosslinker.

15. A method of making a conjugate comprising,

reacting a polysaccharide with a carbonylating agent, thereby forming a polysaccharide having carbamate groups;

and

adding a reactive amino group to each of a plurality of chemical or biomolecules; and

coupling a plurality of the carbamate groups to the plurality of chemical or biomolecules.

16. (canceled)

17. A method of making a conjugate comprising reacting a branched polysaccharide with a heterobifunctional photoreactive crosslinker.

18. A method of making a conjugate comprising reacting a branched polysaccharide with 3-maleimidopropionic acid.

20-21. -21. (canceled)

24. A method of increasing the signal from an array formed on a CMOS image sensor comprising coupling a plurality of probes to a molecule selected from the group consisting of polysaccharides, hydrogels, and branched oligonucleotides.

25. A method of detecting analytes in an array comprising increasing the frame integration time of a CMOS image sensor, thereby integrating analyte signal.

26. (canceled)

27. A sensor device comprising,

an optical digital image sensor;

a low-light enclosure; and

a conjugate probe array.

28. The sensor device of claim 27 wherein the optical digital image sensor is a CMOS image sensor.

29. (canceled)

30. A biosensor system comprising,

a reading station having a first connector;

a portable low-light enclosure comprising;

an optical digital image sensor;

a probe array;

a second connector for attaching the portable enclosure to the first connector, thereby attaching the portable enclosure to the reading station.

31-33. -33. (canceled)

34. An assay kit comprising a polysaccharide conjugate coupled to at least one recognition molecule and at least one signaling molecule.