REDUCTION OF THE TOXIC EFFECT OF IMPURITIES FROM RAW MATERIALS BY EXTRACTIVE FERMENTATION
First Claim
1. A method of improving production of a bioproduct from engineered microbial cells, comprising:
- providing a fermentation broth comprising a crude carbon source;
inoculating said fermentation broth with said microbial cells; and
incubating the inoculated fermentation broth;
wherein said bioproduct is a hydrophobic solvent immiscible with said fermentation broth, and wherein a toxic side product present in said crude carbon source is soluble in said hydrophobic solvent.
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Accused Products
Abstract
There are provided bioproducts and methods of improving production of the bioproducts from engineered microbial cells, the methods comprising: providing a fermentation broth comprising a crude carbon source; inoculating said fermentation broth with said microbial cells; and incubating the inoculated fermentation broth; wherein said bioproduct is a hydrophobic solvent immiscible with said fermentation broth, and wherein a toxic side product present in said crude carbon source is soluble in said hydrophobic solvent. Also, provided are kits for practicing the methods of improving production of bioproducts.
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Citations
102 Claims
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1. A method of improving production of a bioproduct from engineered microbial cells, comprising:
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providing a fermentation broth comprising a crude carbon source; inoculating said fermentation broth with said microbial cells; and incubating the inoculated fermentation broth; wherein said bioproduct is a hydrophobic solvent immiscible with said fermentation broth, and wherein a toxic side product present in said crude carbon source is soluble in said hydrophobic solvent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. A method of passively phase-separating a toxic side product from an aqueous phase of a fermentation broth that is a mixture of crude carbon source and microbial cells engineered to produce a bioproduct during fermentation of a culture of said microbial cells, comprising:
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providing said fermentation broth comprising a crude carbon source; inoculating said fermentation broth with said microbial cells to form a fermentation culture; and incubating said fermentation culture to yield production of said bioproduct; wherein said bioproduct is a hydrophobic solvent, and wherein said toxic side product present in said crude carbon source is soluble in said hydrophobic solvent. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40)
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41. A kit for producing bioproducts from bioengineered cells, comprising:
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a sample of bioengineered cells that are bioengineered to produce a bioproduct from a carbon source; a fermentation broth comprising a crude carbon source derived from conversion of a biomass; a hydrophobic solvent immiscible with said fermentation broth and capable of extracting a toxic side product formed during conversion of said biomass; and instructions for a fermentation process using said sample of bioengineered cells, fermentation broth, and hydrophobic solvent. - View Dependent Claims (42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55)
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56. A continuous fermentation process for the production of a bioproduct from engineered microbial cells, comprising:
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providing a culture of said microbial cells; introducing a continuous flow of volume of a fermentation broth comprising a crude carbon source to said culture; fermenting said culture with said fermentation broth; and removing a continuous flow of volume of said fermentation broth from said culture; wherein said bioproduct is a hydrophobic solvent that is immiscible with said fermentation broth, and wherein a toxic side product present in said crude carbon source is soluble in said hydrophobic solvent. - View Dependent Claims (57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75)
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76. A bioproduct produced by an engineered microbial cell by a process comprising:
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preparing a fermentation broth comprising a crude carbon source; inoculating said fermentation broth with said microbial cells; incubating the inoculated fermentation broth; recovering said bioproduct from the incubated fermentation broth; wherein said bioproduct is a hydrophobic solvent immiscible with said fermentation broth, and wherein a toxic side product present in said crude carbon source is soluble in said hydrophobic solvent. - View Dependent Claims (77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97)
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98. A vehicle powered by energy from combustion of a bioproduct produced by an engineered microbial cell by a process comprising:
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preparing a fermentation broth comprising a crude carbon source; inoculating said fermentation broth with said microbial cells; incubating the inoculated fermentation broth; recovering said bioproduct from the incubated fermentation broth; wherein said bioproduct is a hydrophobic solvent immiscible with said fermentation broth, and wherein a toxic side product present in said crude carbon source is soluble in said hydrophobic solvent. - View Dependent Claims (99, 100, 101, 102)
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Specification