Method of Identifying Hairpin DNA Probes by Partial Fold Analysis

• US 20090087835A1
• Filed: 01/03/2005
• Published: 04/02/2009
• Est. Priority Date: 01/02/2004
• Status: Active Grant

• Current Assignee: University of Rochester
• Original Assignee: University of Rochester
• Inventors: Strohsahl, Christopher M., Miller, Benjamin L.
• Granted Patent: US 7,598,034 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C12Q 1/6813 Hybridisation assays
• CPC Class Codes: C12Q 1/6818 involving interaction of two or more labels, e.g. resonant energy transfer
• CPC Class Codes: C12Q 1/6851 Quantitative amplification
• CPC Class Codes: C12Q 2525/301 Hairpin oligonucleotides
• CPC Class Codes: C12Q 2561/113 Real time assay
• CPC Class Codes: C12Q 2565/1015 labels being on the same oligonucleotide

Abstract

Method of identifying molecular beacons in which a secondary structure prediction algorithm is employed to identify oligonucleotide sequences within a target gene having the requisite hairpin structure. Isolated oligonucleotides, molecular beacons prepared from those oligonucleotides, and their use are also disclosed.

Claims

1. A method of identifying hairpin nucleic acid probes, the method comprising:

providing a target nucleic acid sequence that is larger than about 100 nucleotides in length;

predicting a folded structure of the target nucleic acid sequence;

identifying a nucleotide sequence of a hairpin within the folded structure of the target nucleic acid sequence; and

predicting a folded structure for the identified nucleotide sequence of the hairpin, in the absence of other nucleotides of the target nucleic acid sequence, wherein the folded structure of the hairpin has a predicted E value of at most about −

3 kcal/mol.

2. The method according to claim 1 wherein the nucleotide sequence of the hairpin is between about 12 and about 60 nucleotides in length.

3. The method according to claim 1 wherein the folded structure of the hairpin has a predicted E value of between about −

4 kcal/mol and about −

12 kcal/mol.

4. The method according to claim 1 further comprising:

predicting a folded structure of a duplex formed between the hairpin and its complement.

5. The method according to claim 4 further comprising:

determining whether duplex formation is energetically favorable.

6. The method according to claim 1 further comprising:

performing a database search for nucleotide sequences that are similar to the identified nucleotide sequence of the hairpin.

7. The method according to claim 6 further comprising:

determining, from the results of the performed database search, whether a clear demarcation exists between scores for target nucleic acid sequences and scores for non-target nucleic acid sequences.

8. The method of preparing a molecular beacon comprising:

providing a hairpin nucleic acid probe identified according to the method of claim 1; and

tethering a fluorescent label and a quenching agent to the opposed termini of the provided hairpin nucleic acid probe to form a molecular beacon, wherein the molecular beacon is substantially non-fluorescent in the absence of a nucleic acid complementary to the hairpin nucleic acid probe.

9. The method according to claim 8, wherein said providing comprises:

synthesizing a nucleic acid molecule corresponding to the nucleotide sequence of the hairpin probe.

10. The method according to claim 8, wherein the fluorescent label is tethered to the 5′

terminus and the quenching agent is tethered to the 3′

terminus.

11. The method according to claim 8, wherein the fluorescent label is tethered to the 3′

terminus and the quenching agent is tethered to the 5′

terminus.

12. The method according to claim 8, wherein the quenching agent is a solid surface.

13. The method according to claim 8, wherein the quenching agent is a micro- or nano-particle.

14. The method according to claim 8, wherein the fluorescent label is a fluorescent dye, semiconductor quantum dot, lanthanide atom-containing complex, or fluorescent protein.

15. The method according to claim 8, wherein the quenching agent is a metal or 4-([4-(Dimethylamino)phenyl]azo)benzoic acid.

16. The method according to claim 15, wherein the metal is gold, silver, platinum, copper, cobalt, iron, or iron-platinum.

17. A method of preparing a hairpin nucleic acid molecule comprising:

synthesizing a hairpin nucleic acid molecule identified according to the method of claim 1.

18-30. -30. (canceled)