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Multiplex Digital Immuno-Sensing Using a Library of Photocleavable Mass Tags

  • US 20090088332A1
  • Filed: 11/20/2006
  • Published: 04/02/2009
  • Est. Priority Date: 11/21/2005
  • Status: Abandoned Application
First Claim
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1. A method for detecting the presence of an agent in a sample comprising:

  • (a) contacting the sample with a solid substrate having affixed thereto a first antibody which binds to the agent, wherein the contacting is performed under conditions which would permit the first antibody to bind to the agent if present in the sample;

    (b) removing any unbound sample from the solid substrate;

    (c) contacting the solid substrate with a second antibody which binds to the agent concurrently with the first antibody, wherein the second antibody has a mass tag cleavably affixed thereto, and wherein the contacting is performed under conditions which would permit the second antibody to bind to the agent if present in the sample;

    (d) removing any unbound second antibody;

    (e) cleaving the mass tag from any bound second antibody; and

    (f) detecting the presence of any cleaved mass tag,wherein the presence of cleaved mass tag indicates that the agent is present in the sample.

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