DIFFERENTIATION OF NON-EMBRYONIC STEM CELLS TO CELLS HAVING A PANCREATIC PHENOTYPE
First Claim
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1. A method comprising contacting non-embryonic stem, non-germ, non-embryonic germ cells that can differentiate into at least two of ectodermal, endodermal and mesodermal cell types with a first agent, wherein the first agent is Activin A and a second agent, wherein the second agent inhibits sonic hedgehog (SHH) activity to yield cells having increased expression of Pdx-1.
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Abstract
The invention provides methods for differentiating non-embryonic multipotent stem cells along the pancreatic lineage. The present invention further provides non-embryonic multipotent stem cells and progeny derived therefrom to provide pancreatic cells to a subject.
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Citations
57 Claims
- 1. A method comprising contacting non-embryonic stem, non-germ, non-embryonic germ cells that can differentiate into at least two of ectodermal, endodermal and mesodermal cell types with a first agent, wherein the first agent is Activin A and a second agent, wherein the second agent inhibits sonic hedgehog (SHH) activity to yield cells having increased expression of Pdx-1.
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4-7. -7. (canceled)
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8. A method to differentiate non-embryonic stem, non-germ, non-embryonic germ cells that can differentiate into at least two of ectodermal, endodermal and mesodermal cell types comprising the steps of:
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a) contacting the non-embryonic stem, non-germ and non-embryonic germ cells with a first agent, wherein the first agent is Activin A, b) contacting the cells obtained from step a) with Activin-A and a second agent, wherein the second agent inhibits sonic hedgehog activity; c) contacting the cells obtained from step b) with EGF or HGF; and d) contacting the cells obtained from step c) with one or both of nicotinamide or exendin4, to yield cells expressing insulin. - View Dependent Claims (9, 10)
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11-19. -19. (canceled)
- 20. A composition comprising a first agent, wherein the first agent is Activin-A, a second agent, wherein the second agent inhibits sonic hedgehog and non-embryonic stem, non-germ, non-embryonic germ cells that differentiate into at least two of ectodermal, endodermal and mesodermal cell types.
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23-24. -24. (canceled)
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28-36. -36. (canceled)
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37. A method to provide pancreatic cells to a subject in need thereof comprising:
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a) contacting non-embryonic stem, non-germ, non-embryonic germ cells that can differentiate into at least two of ectodermal, endodermal and mesodermal cell types with a first agent, wherein the first agent is Activin A and a second agent, wherein the second agent inhibits sonic hedgehog (SHH) activity to yield cells having increased expression of Pdx-1; and b) administering the cells having increased expression of Pdx-1 so as to provide pancreatic cells in the subject. - View Dependent Claims (38, 39)
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40-55. -55. (canceled)
Specification