Grading of Breast Cancer
First Claim
1. A method to determine the grade of breast cancer cells in a sample from a subject, said method comprisingassaying said sample for gene expression that is increased in grade III relative to grade I breast cancer, wherein said gene expression is of a gene or genes selected fromRACGAP1 (Rac GTPase activating protein 1, chromosomal location 12p13.2-p13.1),CENPA (centromere protein A (17 kD), chromosomal location 2p24-p21),RRM2 (ribonucleotide reductase M2 polypeptide, chromosomal location 2p25-p24),NEK2 (NIMA (never in mitosis gene a)-related kinase, chromosomal location 1q32.2-q41),BUB1 (budding uninhibited by benzimidazoles 1 (yeast homolog), chromosomal location 2q14), ora combination of RACGAP1, CENPA, RRM2, NEK2, and BUB1;
- andidentifying said sample as containing grade III breast cancer cells.
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Abstract
Methods and compositions for the identification of breast cancer grade signatures are provided. The signature profiles are identified based upon multiple sampling of reference breast tissue samples from independent cases of breast cancer and provide a reliable set of molecular criteria for identification of cells as being in one or more particular stages and/or grades of breast cancer.
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Citations
17 Claims
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1. A method to determine the grade of breast cancer cells in a sample from a subject, said method comprising
assaying said sample for gene expression that is increased in grade III relative to grade I breast cancer, wherein said gene expression is of a gene or genes selected from RACGAP1 (Rac GTPase activating protein 1, chromosomal location 12p13.2-p13.1), CENPA (centromere protein A (17 kD), chromosomal location 2p24-p21), RRM2 (ribonucleotide reductase M2 polypeptide, chromosomal location 2p25-p24), NEK2 (NIMA (never in mitosis gene a)-related kinase, chromosomal location 1q32.2-q41), BUB1 (budding uninhibited by benzimidazoles 1 (yeast homolog), chromosomal location 2q14), or a combination of RACGAP1, CENPA, RRM2, NEK2, and BUB1; - and
identifying said sample as containing grade III breast cancer cells. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method to determine the grade of breast cancer cells in a sample from a subject, said method comprising
assaying said sample for gene expression that is decreased in grade I relative to grade III breast cancer, wherein said gene expression is of a gene or genes selected from RACGAP1 (Rac GTPase activating protein 1, chromosomal location 12p13.2-p13.1), CENPA (centromere protein A (17 kD), chromosomal location 2p24-p21), RRM2 (ribonucleotide reductase M2 polypeptide, chromosomal location 2p25-p24), NEK2 (NIMA (never in mitosis gene a)-related kinase, chromosomal location 1q32.2-q41), BUB1 (budding uninhibited by benzimidazoles 1 (yeast homolog), chromosomal location 2q14), or a combination of RACGAP1, CENPA, RRM2, NEK2, and BUB1; - and
identifying said sample as containing grade I breast cancer cells. - View Dependent Claims (9, 10, 11, 12, 13, 14)
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- 15. A method to determine the grade of breast cancer cells in a sample from a subject, said method comprising assaying said sample for an expression pattern of genes correlated with grade I or grade III breast cancer and identifying said sample as containing grade I or grade III breast cancer cells.
Specification