MASS SPECTROMETRIC METHODS FOR DETECTING MUTATIONS IN A TARGET NUCLEIC ACID
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Abstract
Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and mutations in the molecules are provided.
211 Citations
64 Claims
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1-17. -17. (canceled)
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18. A process for characterizing a target nucleic acid from a biological sample, comprising:
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a) amplifying a target nucleic acid from a biological sample by polymerase chain reaction (PCR); b) ionizing and volatilizing the product of step (a); and c) analyzing (i) a forward strand PCR product by mass spectrometry to determine an observed molecular mass of the forward strand PCR product, or (ii) analyzing a reverse strand PCR product by mass spectrometry to determine an observed molecular mass of the reverse strand PCR product; whereby the target nucleic acid is characterized based on the analysis of (c). - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41)
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42. A process for characterizing a target nucleic acid from a biological sample, comprising:
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a) providing a biological sample; b) isolating a target nucleic acid from the biological sample; c) amplifying the target nucleic acid of (b) by polymerase chain reaction (PCR); d) ionizing and volatilizing the product of step (c); and e) analyzing (i) a forward strand PCR product by mass spectrometry to determine an observed molecular mass of the forward strand PCR product, or (ii) analyzing a reverse strand PCR product by mass spectrometry to determine an observed molecular mass of the reverse strand PCR product; whereby the target nucleic acid is characterized based on the analysis of (e). - View Dependent Claims (43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64)
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Specification