PUFA-PKS Genes From Ulkenia
First Claim
1. PUFA-PKS, comprising at least one of the amino acid sequences represented in SEQ ID No. 6 (ORF 1), 7 (ORF 2), 8 and/or 80 (ORF 3) and have sequences homologous to them with at least 70% sequence homology that have the biological activity of at least one domain of the PUFA-PKS.
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Abstract
The invention relates to genes which are coded for sequences specific to polyketide synthases (PKS). The thus synthesized PKS is characterized by the enzymatic capacity thereof to produce PUFAs (polyunsaturated fatty acids). The invention also relates to the identification of the corresponding DNA-sequences, in addition to the use of said nucleotide sequences for the production of recombined and/or transgenic organisms.
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Citations
42 Claims
- 1. PUFA-PKS, comprising at least one of the amino acid sequences represented in SEQ ID No. 6 (ORF 1), 7 (ORF 2), 8 and/or 80 (ORF 3) and have sequences homologous to them with at least 70% sequence homology that have the biological activity of at least one domain of the PUFA-PKS.
- 4. An amino acid sequence with at least 70% identity with at least 500 directly successive amino acids of the sequences SEQ ID No. 6 (ORF 1), 7 (ORF 2) and 8 and/or 80 (ORF 3) and that has the biological activity of at least one domain of PUFA-PKS.
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11. A recombinant host cell comprising a recombinant DNA construct in which the elements controlling the translation are selected from the group consisting of SEQ ID No. XX-YY (terminators/promoters) or parts thereof from at least 500 nucleotides as well as functional variants of them.
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17. A method of producing artificial polyketides comprising the steps of using individual enzyme domains from the SEQ ID No. 6, 7, 8 and/or 80 comprising PUFA-PKS.
- 18. PUFA-PKS comprising at least one of the amino acid sequences represented in SEQ ID No. 32, 34, 45, 58, 59, 60, 61, 72, 74 and/or 77 and have sequences homologous to them with at least 70% sequence homology and that have the biological activity of at least one domain of the PUFA-PKS.
Specification