METHODS FOR MAKING AND USING SPR MICROARRAYS

• US 20090093067A1
• Filed: 12/08/2008
• Published: 04/09/2009
• Est. Priority Date: 12/06/2005
• Status: Active Grant

• Current Assignee: Suzhou Puxin Life Science & Technology Limited
• Original Assignee: Lumera Corp. (Renesas Electronics Corporation)
• Inventors: Wolf, Nicholas Adam, Barklund, Anna, Dinu, Raluca, Jin, Danliang
• Granted Patent: US 8,094,315 B2
• Time in Patent Office: Days
• US Class Current: 436/501
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/553 Metal or metal coated
• CPC Class Codes: Y10T 428/12493 Composite; i.e., plural, adjacent, spatially distinct metal components [e.g., layers, joint, etc.]
• CPC Class Codes: Y10T 428/12535 with additional, spatially distinct nonmetal component
• CPC Class Codes: Y10T 428/12556 Organic component
• CPC Class Codes: Y10T 428/1259 Oxide
• CPC Class Codes: Y10T 428/12597 Noncrystalline silica or noncrystalline plural-oxide component [e.g., glass, etc.]
• CPC Class Codes: Y10T 428/12861 Group VIII or IB metal-base component
• CPC Class Codes: Y10T 428/31663 As siloxane, silicone or silane
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

An article, process, and method for surface plasmon resonance plates are described. A substrate is covered with a thin metal film onto which a second thin metal film is deposited. The surface of the second thin metal film is converted to the metal oxide which is used to covalently bond organosilanes to the surface. Reactive organosilanes containing terminal bonding groups are arranged in a plurality of spots that are surrounded by inert organosilanes. Biomolecule attachment to the binding group is detected or measured from surface plasmon signals from the first thin metal film.

Claims

1-20. -20. (canceled)

21. A process, comprising:

a) depositing a first metal film on a substrate;

b) depositing a second thin metal film on the first metal film;

c) converting the upper surface of the second thin metal film to the corresponding metal oxide; and

d) reacting a functionalized organosilane with the metal oxide, whereby a reactive organosilane is formed on the upper surface of the second thin metal film.

22. The process of claim 21, wherein the first metal film is gold, silver, or copper and the second thin metal film is Ti, Zr, Al, Cr, Hf, V, Ta, W, or Pb.

23. The process of claim 21, wherein the substrate is glass or quartz.

24. The process of claim 21, wherein converting the upper surface of the second thin metal film to the corresponding metal oxide comprises oxygen plasma treatment.

25. The process of claim 21, wherein the functionalized organosilane is

wherein:

R³ is chlorine or alkoxy;

p=1-20; and

the reactive organosilane comprises

26. The process of claim 25, wherein the reactive organosilane is

and the process further comprises converting the reactive organosilane to

where m=1-10.

27. The process of claim 21, further comprising patterning the reactive organosilane into a plurality of spots.

28. The process of claim 21, wherein the process further comprises reacting the reactive organosilane with DNA or RNA.

29. A process comprising:

a) depositing a first metal film on a substrate;

b) depositing a second thin metal film on the first metal film;

c) converting the upper surface of the second thin metal film to the corresponding metal oxide;

d) reacting an inert organosilane with the metal oxide;

e) patterning the inert organosilane to give a pattern of a plurality of metal oxide spots surrounded by inert organosilane; and

f) reacting a functionalized organosilane with the metal oxide, whereby a reactive organosilane is formed in the metal oxide spots on the second thin metal film.

30. The process of claim 29, wherein the inert organosilane includes a fluorinated alkyl group.

31. The process of claim 30, wherein the inert organosilane comprises

wherein:

q=1-4; and

p=1-20.

32. The process of claim 29, wherein the functionalized organosilane is

wherein:

R³ is chlorine or alkoxy;

p=1-20; and

the reactive organosilane comprises

33. The process of claim 32, wherein the reactive organosilane is

and the process further comprises converting the reactive organosilane to

where m=1-10.

34. The process of claim 29, wherein the size of each spot is 10-1000 μ

m in diameter.

35. The process of claim 29, wherein the density of spots is 100 to 1 million per square centimeter.

36. A method, comprising a) providing an article including:

i) a substrate;

ii) a first metal film overlying the substrate;

iii) a second thin metal film including metal-oxide bonds overlying the first metal film;

iv) an organosilane covalently bonded to the exposed upper surface of the second thin metal film through metal-oxygen-silicon bonds; and

v) a binding group attached to the organosilane; and

b) determining whether or not the biomolecule is bound to the binding group by detecting or measuring a surface plasmon resonance signal generated from the first metal film.

37. The method of claim 36, wherein the binding group comprises DNA or RNA.

38. The method of claim 37, wherein the biomolecule is DNA, RNA, or a protein.

39. The method of claim 36, wherein the binding group is an antigen, an antibody, or a hapten.

40. The method of claim 36, wherein the organosilane has a pattern of a plurality of spots.

41. The method of claim 40, wherein the spots are arranged in a microarray.

42. The method of claim 41, wherein the size of each spot is 10-1000 μ

m in diameter.

43. The method of claim 41, wherein the density of spots is 100 to 1 million per square centimeter.

44. The method of claim 36, wherein the first metal film is gold, silver, or copper and the second thin metal film is Ti, Zr, Al, Cr, Hf, V, Ta, W, or Pb.

45. The method of claim 36, wherein the substrate is glass or quartz.