Bio-polymer and scaffold-sheet method for tissue engineering
First Claim
1. A scaffold polymer for tissue engineering comprising:
- a crosslinked urethane-containing polyester.
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Abstract
A method of making a new type of biomaterials, biodegrable crosslinked urethane-containing polyester (CUPE) elastomers and a scaffold-sheet engineering method for tissue engineering applications is provided. CUPEs can be synthesized by forming a linear pre-polymer, which is a polyester, introducing the urethane bonds into polyester using a diisocyanate as a linker, and crosslinking the resulting urethane containing linear polymers to form CUPEs via post-polymerization. This family of polymers, CUPEs, exhibit excellent biocompatibility with desired degradation. Tissue engineering scaffolds made of CUPEs are soft and elastic, and have good mechanical strength. Complex tissue grafts can be constructed by a novel layer-by-layer (LBL) scaffold-sheet engineering design using CUPE sheets. CUPE scaffolds can provide openings for cell to cell communication across scaffold layers and angiogenesis into the depth of the construct. Biomolecules, such as anticoagulants, can be incorporated into the CUPE polymers, increasing their viability as vascular graft scaffolds.
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Citations
23 Claims
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1. A scaffold polymer for tissue engineering comprising:
a crosslinked urethane-containing polyester. - View Dependent Claims (2)
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3. A method of synthesizing a biomaterial, the method comprising:
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adding citric acid and 1,8 octane diol in a molar ratio of 1;
1.1 to a flask, forming a mixture;melting the mixture at a melting temperature; allowing the mixture to react, creating a pre-polymer; dissolving the pre-polymer in dioxane; precipitating a polymer in water, removing any unreacted monomers and oligomers; freeze drying the polymer; dissolving the freeze dried polymer in N,N′
-dimethylformamide;adding 1,6-hexane diisocyanate (HDI) in about a 1;
0.9, prepolymer to HDI ratio, under continuous stirring to form pre-crosslinked urethane-containing polyester (CUPE) solution, by introducing urethane linkage in the polymer;assessing reaction completeness using Fourier Transform Infra Red Spectroscopy (FT-IR); determining reaction completion by an absence of isocyanate group in the FT-IR; purifying pre-CUPE solution by dropping the pre-CUPE solution into water while stirring; drying the purified pre-CUPE under vacuum; and heating pre-CUPE at a temperature in a range of 60-120°
C. for a time in a range of one day to 2 weeks with or without vacuum in an oven to obtain CUPE polymers, the biomaterial.
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4. A small diameter blood vessel graft comprising:
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a CUPE tubular scaffold; at least one HASMC seeded CUPE scaffold sheet, which is wrapped around the CUPE tubular scaffold; at least one HAFB seeded CUPE scaffold sheet, which is wrapped around the at least one wrapped HASMC seeded scaffold sheet; and HAECs seeded in the lumen of the CUPE tubular scaffold.
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5. A method of synthesizing a biomaterial polymer, the method comprising:
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forming linear pre-polymers, which are polyester; using a diisocyanate as a linker; introducing urethane bonds into the formed polyester, yielding urethane-containing linear polymers; crosslinking the resulting urethane containing linear polymers to form CUPEs via post-polymerization. - View Dependent Claims (6, 7, 8, 9, 10, 23)
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11. A method of synthesizing a biomaterial polymer, the method comprising:
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forming pre-polymers via polycondensation, using multifunctional monomers; using a diisocyanate as a linker; introducing urethane bonds into the formed pre-polymers, yielding urethane-containing linear polymers, pre-CUPEs; crosslinking the resulting the urethane-containing linear pre polymers to form CUPEs via post-polymerization. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification