Method for synthesizing DNA
First Claim
1. A DNA synthesis reaction composition comprising:
- 1) a DNA polymerase having 3′
→
5′
exonuclease activity;
2) water-soluble acidic macromolecular substances selected from the group consisting of carrageenan, rhamnan sulfate, chondroitin sulfate, dermatan sulfate, hyaluronic acid, pectin, polyvinyl sulfates, polystyrene sulfates, polyglutamic acids, polyacrylic acids, and water-soluble salts thereof; and
3) components necessary for DNA synthesis using DNA polymerase.
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Abstract
A DNA synthesis reaction-enhancer comprising at least one kind selected from the group consisting of acidic substances and cationic complexes; a DNA synthesis method in which during a DNA synthesis reaction a reaction is carried out in the presence of the above enhancer by using DNA polymerase; a DNA synthesis reaction composition comprising the above enhancer; a DNA synthesis reaction composition comprising two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity; a DNA synthesis method in which during a DNA synthesis reaction two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity are used; a kit for use in in vitro DNA synthesis, comprising two or more kinds of DNA polymerases each having 3′→5′ exonuclease activity; and a kit for use in in vitro DNA synthesis, wherein the kit comprises the DNA synthesis reaction-enhancer and DNA polymerase. According to the present invention, DNA synthesis can be carried out at an efficiency more excellent as compared to conventional DNA synthesis reaction.
30 Citations
7 Claims
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1. A DNA synthesis reaction composition comprising:
-
1) a DNA polymerase having 3′
→
5′
exonuclease activity;2) water-soluble acidic macromolecular substances selected from the group consisting of carrageenan, rhamnan sulfate, chondroitin sulfate, dermatan sulfate, hyaluronic acid, pectin, polyvinyl sulfates, polystyrene sulfates, polyglutamic acids, polyacrylic acids, and water-soluble salts thereof; and 3) components necessary for DNA synthesis using DNA polymerase. - View Dependent Claims (2, 3, 4)
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5. A kit for use in in vitro DNA synthesis, comprising:
-
1) DNA polymerases having 3′
→
5′
exonuclease activity;2) water-soluble acidic macromolecular substances selected from the group consisting of carrageenan, rhamnan sulfate, chondroitin sulfate, dermatan sulfate, hyaluronic acid, pectin, polyvinyl sulfates, polystyrene sulfates, polyglutamic acids, polyacrylic acids, and water-soluble salts thereof; and 3) components necessary for DNA synthesis using DNA polymerase. - View Dependent Claims (6, 7)
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Specification