Liquid end assembly for a handheld multichannel pipette with adjustable nozzle spacing

• US 20090104079A1
• Filed: 10/17/2007
• Published: 04/23/2009
• Est. Priority Date: 10/17/2007
• Status: Active Grant

• Current Assignee: Rainin Instrument LLC
• Original Assignee: Rainin Instrument LLC
• Inventors: Vitale, Nicholas R., O'Connell, Kenneth P.
• Granted Patent: US 7,947,234 B2
• Time in Patent Office: Days
• US Class Current: 422/100
• CPC Class Codes: B01L 2200/022 Variable spacings
• CPC Class Codes: B01L 2300/027 Digital display, e.g. LCD, LED
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/0217 of the plunger pump type medical syringes A61M
• CPC Class Codes: B01L 3/0237 Details of electronic control, e.g. relating to user interface

Abstract

A handheld multichannel pipette includes multiple fluid-handling nozzles and a rotary adjustment mechanism for adjusting the width of a pattern of nozzles while maintaining equal spacing between pairs of nozzles. Embodiments of the adjustable-spacing multichannel pipette include an adjustable stop mechanism.

Claims

1. A liquid-end assembly for a handheld multichannel pipette, the liquid-end assembly comprising:

a housing for the liquid-end assembly, wherein the housing is configured to receive a plunger shaft connectable to a drive mechanism of the pipette for axial movement of the plunger shaft in the housing;

a plurality of cylinders mounted within the housing;

a plurality of air displacement pistons each mounted for axial movement in and through an open upper end of one of the cylinders in response to axial movement of the shaft in the housing; and

a plurality of nozzles, each connected to a respective one of the plurality of cylinders, and each with a lower open end extending from a bottom wall of the housing; and

a spacing adjustment mechanism configured to be manipulated by a user and to displace at least one nozzle from another relative to rotation of an adjustment component.

2. The liquid-end assembly for a handheld multichannel pipette of claim 1, wherein the spacing adjustment mechanism is configured to maintain a uniform spacing between adjacent pairs of the plurality of nozzles.

3. The liquid-end assembly for a handheld multichannel pipette of claim 1, wherein each of the plurality of nozzles is configured to receive a disposable pipette tip.

4. The liquid-end assembly for a handheld multichannel pipette of claim 3, wherein the liquid end assembly includes a plurality of fluid-tight pathways between each of the plurality of cylinders and a corresponding tip coupled to a corresponding nozzle.

5. The liquid-end assembly for a handheld multichannel pipette of claim 4, wherein movement of a piston within a cylinder of the plurality of cylinders causes a corresponding movement of an air column between the piston and the lower open end of the corresponding nozzle.

6. The liquid-end assembly for a handheld multichannel pipette of claim 1, wherein the cylinders have a fixed spacing not responsive to an actuation of the adjustment component, and wherein the liquid end assembly further comprises a plurality of flexible hoses coupling each cylinder of the plurality of cylinders to a corresponding nozzle.

7. The liquid-end assembly for a handheld multichannel pipette of claim 6, further comprising a manifold coupled to the plurality of cylinders and coupling each of the cylinders to one of the plurality of flexible hoses.

8. The liquid-end assembly for a handheld multichannel pipette of claim 1, wherein at least one cylinder of the plurality of cylinders moves with a corresponding nozzle of the plurality of nozzles when the adjustment mechanism is manipulated.

9. The liquid-end assembly for a handheld multichannel pipette of claim 1, wherein the adjustment component of the spacing adjustment mechanism comprises a rotatable nozzle spacing cam.

10. The liquid-end assembly for a handheld multichannel pipette of claim 9, wherein the nozzle spacing cam has a plurality of helical grooves.

11. The liquid-end assembly for a handheld multichannel pipette of claim 10, wherein:

The nozzle spacing cam has a centerpoint;

the grooves are substantially symmetric about the centerpoint;

each subsequent groove on either side of the centerpoint increases in pitch; and

each nozzle of the plurality of nozzles corresponds to a groove of the plurality of grooves on the nozzle spacing cam.

12. The liquid-end assembly for a handheld multichannel pipette of claim 10, wherein each nozzle of the plurality of nozzles tracks the corresponding groove on the nozzle spacing cam as the nozzle spacing cam rotates.

13. The liquid-end assembly for a handheld multichannel pipette of claim 12, wherein each nozzle of the plurality of nozzles is restricted from rotating with the nozzle spacing cam by engagement with a nozzle rail parallel to the nozzle spacing cam.

14. The liquid-end assembly for a handheld multichannel pipette of claim 13, wherein rotation of the nozzle spacing cam causes each nozzle of the plurality of nozzles to traverse axially along the nozzle spacing cam and to slide along the nozzle rail.

15. The liquid-end assembly for a handheld multichannel pipette of claim 10, wherein:

one nozzle of the plurality of nozzles is stationary;

starting from an end of the spacing adjustment cam adjacent to the stationary nozzle, each successive groove on the spacing adjustment cam increases in pitch; and

each remaining nozzle of the plurality of nozzles corresponds to a groove of the plurality of grooves on the nozzle spacing cam.

16. The liquid-end assembly for a handheld multichannel pipette of claim 15, wherein each remaining nozzle of the plurality of nozzles tracks the corresponding groove on the nozzle spacing cam as the nozzle spacing cam rotates.

17. The liquid-end assembly for a handheld multichannel pipette of claim 16, wherein each remaining nozzle of the plurality of nozzles is restricted from rotating with the nozzle spacing cam by engagement with a nozzle rail parallel to the nozzle spacing cam.

18. The liquid-end assembly for a handheld multichannel pipette of claim 17, wherein rotation of the nozzle spacing cam causes each remaining nozzle of the plurality of nozzles to traverse axially along the nozzle spacing cam and to slide along the nozzle rail.

19. The liquid-end assembly for a handheld multichannel pipette of claim 9, wherein the nozzle spacing cam has a plurality of helical lobes.

20. The liquid-end assembly for a handheld multichannel pipette of claim 19, wherein at least one nozzle of the plurality of nozzles tracks a corresponding lobe on the nozzle spacing cam.

21. The liquid-end assembly for a handheld multichannel pipette of claim 9, wherein the spacing adjustment mechanism further includes a spacing adjustment knob, and wherein the nozzle spacing cam is coupled to the spacing adjustment knob.

22. The liquid-end assembly for a handheld multichannel pipette of claim 1, wherein the pipette further comprises a stop adjustment mechanism configurable by a user to set a maximum nozzle spacing.

23. The liquid-end assembly for a handheld multichannel pipette of claim 22, wherein the stop adjustment mechanism comprises a stop knob operative to set an angular position of a stop ledge corresponding to a desired maximum nozzle spacing.

24. The liquid-end assembly for a handheld multichannel pipette of claim 23, wherein the spacing adjustment mechanism comprises a stop component coupled to rotate with the adjustment component, and wherein the stop component carries a stop tab positioned to strike the stop ledge of the stop knob and to restrict the rotation of the adjustment component when a spacing of the nozzles has reached the desired maximum nozzle spacing.