Preparation of Molecular Imprinted Polymers
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Abstract
One aspect is a method for improved preparation of molecular imprinted polymer (MIP) particles, where initial compositions comprising insoluble MIP particles are enriched for those MIP particles that bind a particular target molecule, thus excluding non-binding and weakly binding particles from the final composition. Enrichment is typically accomplished via use of chromatographic methods capable of separating particulate material or by means of agglutination. Another aspect is preparation of improved insoluble MIPs by use of extended micronization of raw MIP particles with a view to expose a large number of binding sites per mass unit of MIP particles. In preferred embodiments the two aspects are combined. The resulting improved MIPs may be used for diagnostic, analytical and therapeutic purposes, notably as orally administered drugs which can bind substances such as cholesterol and bile acids and bile acid salts in the gastrointestinal tract.
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Citations
61 Claims
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1-28. -28. (canceled)
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29. A method of preparing a composition comprising molecular imprinted polymers having high binding capacity and specificity for a target molecule, said method comprising:
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a) obtaining a suspension of insoluble molecular imprinted polymers, which bind the target molecule, and which have been prepared using the target molecule or a mimic thereof as template molecule, b) subjecting the suspended molecular imprinted polymers to an affinity purification procedure, wherein the template molecule or a fragment thereof or a mimic thereof is used as capture agent, c) recovering the molecular imprinted polymers that bind the capture agent in the affinity purification procedure while substantially excluding the capture agent and molecular imprinted polymers that do not bind the capture agent from the recovered product, and d) combining the molecular imprinted polymers recovered and optionally a carrier, vehicle or diluent to obtain said composition. - View Dependent Claims (30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 52, 53, 61)
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- 49. A composition of insoluble molecular imprinted polymers wherein substantially all molecular imprinted polymers in the composition bind the same target molecule and, optionally, the composition does not include all binding sites for the target molecule.
- 56. A method for preparing a pharmaceutical for the treatment, prophylaxis or amelioration of cardiovascular disease, hypertension, atherosclerosis, gallstone disease, cholestatic liver disease, hypercholesterolemia, obesity, infections originating from parasites, virus or micro organisms such as bacteria and fungi, or poisoning originating from orally received toxins, said method comprising combining insoluble molecular imprinted polymers wherein substantially all molecular imprinted polymers in the composition bind the same target molecule and, optionally, the composition does not include all binding sites for the target molecule with a pharmaceutically acceptable excipient.
Specification