METHOD FOR DETECTING A TARGET NUCLEIC ACID SEQUENCE
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Abstract
A method of detecting a target nucleic acid sequence comprising providing a stem-and-loop structured nucleic acid for measurement wherein the nucleic acid comprises complementary sequence portions located at both terminals and a target sequence portion therebetween as well as a double-stranded portion formed by hybridization of the complementary sequence portions located at both terminals and a remaining looped single-stranded portion, providing a probe nucleic acid having a sequence complementary to the target sequence portion wherein one end of the probe nucleic acid being immobilized to a solid substrate surface, reacting the nucleic acid for measurement with the probe nucleic acid to specifically hybridize the target sequence portion of the nucleic acid for measurement to the probe nucleic acid, and detecting presence or absence of the nucleic acid for measurement hybridized to the probe nucleic acid.
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Citations
28 Claims
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1-18. -18. (canceled)
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19. A method of detecting a target nucleic acid sequence comprising:
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providing a stem-and-loop structured nucleic acid for measurement wherein the nucleic acid comprises complementary sequence portions located at both terminals and a target sequence portion therebetween as well as a double-stranded portion formed by hybridization of the complementary sequence portions located at both terminals and a remaining looped single-stranded portion; providing a probe nucleic acid having a sequence complementary to the target sequence portion wherein one end of the probe nucleic acid being immobilized to a solid substrate surface; reacting the nucleic acid for measurement with the probe nucleic acid to specifically hybridize the target sequence portion of the nucleic acid for measurement to the probe nucleic acid; and detecting presence or absence of the nucleic acid for measurement hybridized to the probe nucleic acid, thereby detecting the target nucleic acid sequence; wherein 5′
to 3′
sequence orientations of both the probe nucleic acid and the target sequence portion are arranged so that the double-stranded portion of the nucleic acid for measurement extends away from the solid surface when the probe nucleic acid and the target sequence portion is hybridized. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26)
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- 27. A LAMP product amplified by LAMP method for detection of the target sequence, wherein the target sequence is inserted between F1 region and F2 region, between F2c region and F1c region, between B1 region and B2 region, and/or between B2c region and B1c region of the LAMP product.
Specification