Method and Apparatus for Assaying Blood Clotting
First Claim
1. An apparatus for assaying clotting activity comprising:
- an inlet for a blood fluid;
a vessel in fluid communication with the inlet; and
at least a first and a second patch in the vessel, wherein(a) the patches each comprise stimulus material which is capable of initiating a clotting pathway when contacted with a blood fluid from a subject; and
(b)(i) the stimulus material in the first patch differs from the second patch;
or(b)(ii) the concentration of stimulus material in the first patch differs from the second patch;
or(b)(iii) the first patch has a surface area different from the second patch;
or(b)(iv) the first patch has a shape different from the second patch;
or(b)(v) the first patch has a size different from the second patch.
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Accused Products
Abstract
This invention provides an apparatus for assaying clotting activity. The apparatus includes an inlet for a blood fluid and two or more patches of material in the vessel. The material is capable of initiating a clotting pathway in a blood fluid. This invention also provides an apparatus for measuring clot propagation, which includes a region with material capable of initiating a clotting pathway, and a region where the clot propagation is monitored. Also provided are methods for assaying clotting activity, assaying the integrity of a blood clotting pathway, assaying the effect of a substance on the integrity of a blood clotting pathway, monitoring clot propagation, and preventing clot propagation from one vessel to another.
45 Citations
37 Claims
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1. An apparatus for assaying clotting activity comprising:
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an inlet for a blood fluid; a vessel in fluid communication with the inlet; and at least a first and a second patch in the vessel, wherein (a) the patches each comprise stimulus material which is capable of initiating a clotting pathway when contacted with a blood fluid from a subject; and (b)(i) the stimulus material in the first patch differs from the second patch;
or(b)(ii) the concentration of stimulus material in the first patch differs from the second patch;
or(b)(iii) the first patch has a surface area different from the second patch;
or(b)(iv) the first patch has a shape different from the second patch;
or(b)(v) the first patch has a size different from the second patch. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method of assaying blood clotting, comprising
contacting blood fluid from a subject with at least a first and second patch, wherein (a) the patches each comprise stimulus material which is capable of initiating a clotting pathway when contacted with a blood fluid from a subject; - and
(b)(i) the stimulus material in the first patch differs from the second patch;
or(b)(ii) the concentration of stimulus material in the first patch differs from the second patch;
or(b)(iii) the first patch has a surface area different from the second patch;
or(b)(iv) the first patch has a shape different from the second patch;
or(b)(v) the first patch has a size different from the second patch; and determining which patches initiate clotting of the blood fluid from the subject. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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31. An apparatus for measuring clot propagation comprising:
a first region comprising a stimulus material; and
a second region in communication with the first region suitable for monitoring the propagation of a clot;
wherein when a blood fluid is placed in the first region, a clot forms and propagates to the second region.- View Dependent Claims (32, 33, 34, 35, 36)
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37. A method of monitoring clot propagation, comprising the steps of:
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contacting a blood fluid with a first region of an apparatus, the first region comprising a stimulus material, and monitoring clot propagation in a second region of the apparatus, the second region in communication with the first region.
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Specification