Apparatus for Thin-Layer Cell Smear Preparation and In-situ Hybridization

• US 20090104686A1
• Filed: 10/21/2007
• Published: 04/23/2009
• Est. Priority Date: 10/21/2007
• Status: Active Grant

• Current Assignee: King Car Food Industrial Company Limited
• Original Assignee: King Car Food Industrial Company Limited
• Inventors: Lee, Bor-Heng, Lin, Ching-Yu, Yan, Chiu-Cho, Wang, Ying-Chih
• Granted Patent: US 7,906,317 B2
• Time in Patent Office: Days
• US Class Current: 435/285.100
• CPC Class Codes: A61B 10/0045 Devices for taking samples of body liquids devices for taking blood samples A61B5/15
• CPC Class Codes: A61B 10/0096 Casings for storing test samples A61B10/0038 and A61B10/0045 take precedence; preservation of living parts of the human or animal body A01N1/02; containers for retaining a material to be analysed B01L3/50; containers for enzymology or microbiology C12M1/16; swab-sampler being part of enzymology or microbiology container C12M1/30
• CPC Class Codes: A61B 2010/0074 Vaginal or cervical secretions
• CPC Class Codes: Y10S 435/803 Physical recovery methods, e.g. chromatography, grinding
• CPC Class Codes: Y10S 435/805 Test papers

Abstract

The present invention discloses an apparatus for thin-layer cell smear preparation and in-situ hybridization, comprising at least one positioning device and a sealing device. The positioning device comprises at least one first opening. When the positioning device is set on a carrier device, the wall of the first opening and the carrier device form a cavity. The cavity is used to accommodate a cell suspension. The sealing device is provided on the positioning device for sealing the cavity to form an enclosed space.

Claims

1. An apparatus for thin-layer cell smear preparation and in-situ hybridization, the apparatus comprising:

at least one positioning device comprising at least one first opening wherein the wall of said first opening and a carrier device form a cavity when said positioning device is set on said carrier device and said cavity is for accommodating a cell suspension; and

a sealing device provided on said positioning device for sealing said cavity to form an enclosed space.

2. The apparatus according to claim 1, wherein said positioning device comprises a structure with five layers, sequentially from top to bottom, a first release later, a first adhesive layer, a first matrix foam layer, a second adhesive layer, and a second release layer.

3. The apparatus according to claim 2, wherein said second release layer is to protect said second adhesive layer and said second adhesive layer is adhered to said carrier device if said second release layer is removed.

4. The apparatus according to claim 2, wherein said first release layer is to protect said first adhesive layer and said first adhesive layer is adhered to said sealing device if said first release layer is removed.

5. The apparatus according to claim 2, wherein said first matrix foam layer has a thickness of about 0.05 mm˜

5 mm.

6. The apparatus according to claim 2, wherein said first matrix foam layer is selected from the group consisting of the following or any combination thereof:

polyurethane (PU), polyvinylchloride (PVC), polyethylene (PE), polyethylene terephthalate (PET), and neoprene acrylic.

7. The apparatus according to claim 2, wherein said first adhesive layer and said second adhesive layer are made of acrylic adhesive.

8. The apparatus according to claim 1, wherein the shape of said first opening is selected from the group consisting of the following:

circular, square, triangular, wedged or any geometrical shape.

9. The apparatus according to claim 1, wherein said sealing device has a thickness of about 0.05 mm˜

2 mm.

10. The apparatus according to claim 1, wherein said carrier device is a slide.

11. The apparatus according to claim 2, further comprising:

a thickening device provided on said positioning device and comprising at least one second opening wherein said second opening is overlapped with said first opening when said thickening device is coupled to said positioning device.

12. The apparatus according to claim 11, wherein said thickening device comprises a structure with two layers, a second matrix foam layer and a third adhesive layer, and said third adhesive layer is adhered to said first release layer.

13. The apparatus according to claim 12, wherein said second matrix foam layer has a thickness of about 0.05 mm˜

5 mm.

14. The apparatus according to claim 12, wherein said second matrix foam layer is selected from the group consisting of the following or any combination thereof:

polyurethane (PU), polyvinylchloride (PVC), polyethylene (PE), polyethylene terephthalate (PET), and neoprene acrylic.

15. The apparatus according to claim 12, wherein said third adhesive layer is made of acrylic adhesive.

16. The apparatus according to claim 1, wherein the method for thin-layer cell smear preparation and in-situ hybridization comprises:

providing said carrier device;

performing a coating procedure to coat a cross-linking agent on a surface of said carrier device wherein the cavity is formed by setting said positioning device on said carrier device after said cross-linking agent is dried;

performing a sample preparation procedure to prepare a cell suspension from specimen cells;

performing a setting procedure to add said cell suspension into said cavity and to have said cross-linking agent adsorb the specimen cell precipitate in said cell suspension to form said thin-layer cell smear; and

performing an in-situ hybridization procedure to add a probe and seal said cavity by said sealing device via a sealing procedure to form an enclosed space so as to carry out said in-situ hybridization.