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Methods and Compositions for Feeder-Free Pluripotent Stem Cell Media Containing Human Serum

  • US 20090104696A1
  • Filed: 10/19/2007
  • Published: 04/23/2009
  • Est. Priority Date: 10/19/2007
  • Status: Active Grant
First Claim
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1. A feeder-free primate pluripotent stem cell tissue culture composition comprising:

  • a) an undifferentiated human embryonic stem cell (hESC);

    b) a defined culture media comprising human serum (hS) or a soluble attachment component thereof, wherein the human serum further comprises at least one soluble attachment component having at least 100 kDa molecular weight, and wherein the composition is essentially free of feeder cells.

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