Methods and Compositions for Feeder-Free Pluripotent Stem Cell Media Containing Human Serum
First Claim
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1. A feeder-free primate pluripotent stem cell tissue culture composition comprising:
- a) an undifferentiated human embryonic stem cell (hESC);
b) a defined culture media comprising human serum (hS) or a soluble attachment component thereof, wherein the human serum further comprises at least one soluble attachment component having at least 100 kDa molecular weight, and wherein the composition is essentially free of feeder cells.
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Abstract
The present invention provides compositions and methods for the culture and maintenance of pluripotent stem cells. More particularly, the present invention provides for compositions and methods for culturing, maintaining, growing and stabilizing primate pluripotent stem cells in a feeder-free defined media further comprising human serum, or a soluble attachment component of the human serum, for promoting cell attachment.
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Citations
14 Claims
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1. A feeder-free primate pluripotent stem cell tissue culture composition comprising:
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a) an undifferentiated human embryonic stem cell (hESC); b) a defined culture media comprising human serum (hS) or a soluble attachment component thereof, wherein the human serum further comprises at least one soluble attachment component having at least 100 kDa molecular weight, and wherein the composition is essentially free of feeder cells. - View Dependent Claims (2, 3, 4, 5)
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6. A method of culturing primate embryonic stem cells in a feeder-free media comprising:
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culturing the primate embryonic stem cells in a culture medium which can support stem cells, the culture medium being essentially free of feeder cells, and wherein the medium comprises human serum or a soluble attachment component thereof, thereby promoting undifferentiated embryonic stem cell proliferation. - View Dependent Claims (7, 8, 9, 10, 11, 12, 13)
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14. A method of culturing primate embryonic stem cells in a feeder-free defined media comprising:
culturing the primate embryonic stem cells in a culture medium which can support stem cells, the culture medium being substantially free of feeder cells and further comprising about 0.5% to about 2% human serum or a soluble attachment component thereof the culturing step being conducted for over one month with the embryonic stem cells proliferating in culture while maintaining the potential of the stem cells to differentiate into derivatives of endoderm, mesoderm, and ectoderm tissues, and while maintaining the karyotype of the stem cells.
Specification