SELF-ACTUATING SIGNAL PRODUCING DETECTION DEVICES AND METHODS
First Claim
1. An assay system for the detection of a target in a liquid sample, wherein said assay system comprises:
- a first capture moiety which binds to any target present in said sample, said first capture moiety complexed with an enzymatic redox reaction component which enzyme is a an oxidase or dehydrogenase of a substrate;
a second capture moiety which bind to any said target present in said sample in a way not to interfere with binding of said first capture moiety to said target, said second capture moiety being bound to a an element that facilitates separation of target bound thereby from the remainder of said sample;
a substrate recognized by said enzyme, which substrate, when acted upon by said enzyme, releases electrons; and
a circuit which detects the presence of electric potential, electric current, or both, developed by the release of electrons from said substrate when digested by said enzyme.
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Accused Products
Abstract
An assay system is provided of great sensitivity and portability where the presence of a specific target in a sample, as well as its concentration (qualification and quantification) is detected by reason of a potential or voltage in a closed circuit, built up a redox reaction. The reaction is produced by binding a capture moiety to an enzymatic redox reaction partner, allowing the capture moiety to bind to any target in the sample. In a homogenous assay, the method further comprises washing any such bound target. The bound target may be immobilized through use of a second capture moiety. Substrate for the enzyme is then added. The action on the substrate by the enzyme generates electrons, creating a potential across an anode and cathode which may be separated by a membrane.
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Citations
27 Claims
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1. An assay system for the detection of a target in a liquid sample, wherein said assay system comprises:
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a first capture moiety which binds to any target present in said sample, said first capture moiety complexed with an enzymatic redox reaction component which enzyme is a an oxidase or dehydrogenase of a substrate; a second capture moiety which bind to any said target present in said sample in a way not to interfere with binding of said first capture moiety to said target, said second capture moiety being bound to a an element that facilitates separation of target bound thereby from the remainder of said sample; a substrate recognized by said enzyme, which substrate, when acted upon by said enzyme, releases electrons; and a circuit which detects the presence of electric potential, electric current, or both, developed by the release of electrons from said substrate when digested by said enzyme. - View Dependent Claims (2, 3, 4, 6, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22, 23, 24, 25, 26, 27)
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5. An assay system for the detection of a target in a sample, wherein said assay system comprises:
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a first capture moiety which binds to any target present in said sample, said first capture moiety complexed with an enzymatic redox reaction component which enzyme is a an oxidase or dehydrogenase of a substrate; a substrate recognized by said enzyme, which substrate, when acted upon by said enzyme, releases electrons; and a circuit which detects the presence of electric potential, electric current, or both, developed by the release of electrons from said substrate when acted upon by said enzyme. - View Dependent Claims (7, 8, 9, 21)
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Specification