Detection of Group B Streptococcus
First Claim
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1. An article of manufacture, comprising a pair of pts primers, a pair of pts probes, a donor fluorescent moiety and a corresponding acceptor fluorescent moiety, wherein said pair of pts primers comprises a first pts primer and a second pts primer, wherein said first pts primer consists of the sequence 5′
- -TGA GAA GGC AGT AGA AAG CTT AG-3′
(SEQ ID NO;
1), and wherein said second pts primer consists of the sequence 5′
-TGC ATG TAT GGG TTA TCT TCC-3′
(SEQ ID NO;
2), wherein said pair of pts probes comprises a first pts probe and a second pts probe, wherein said first pts probe consists of the sequence 5′
-CAA ATT AAA GAG ACT ATT CGT GCA A-3′
(SEQ ID NO;
3), and wherein said second pts probe consists of the sequence 5′
-CAA GTA AAT GCA GAA ACA GG-3′
(SEQ ID NO;
4).
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Abstract
The invention provides methods to detect group B streptococcus (GBS) in biological samples using real-time PCR. Primers and probes for the detection of GBS are provided by the invention. Articles of manufacture containing such primers and probes for detecting GBS are further provided by the invention.
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Citations
3 Claims
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1. An article of manufacture, comprising a pair of pts primers, a pair of pts probes, a donor fluorescent moiety and a corresponding acceptor fluorescent moiety, wherein said pair of pts primers comprises a first pts primer and a second pts primer, wherein said first pts primer consists of the sequence 5′
- -TGA GAA GGC AGT AGA AAG CTT AG-3′
(SEQ ID NO;
1), and wherein said second pts primer consists of the sequence 5′
-TGC ATG TAT GGG TTA TCT TCC-3′
(SEQ ID NO;
2), wherein said pair of pts probes comprises a first pts probe and a second pts probe, wherein said first pts probe consists of the sequence 5′
-CAA ATT AAA GAG ACT ATT CGT GCA A-3′
(SEQ ID NO;
3), and wherein said second pts probe consists of the sequence 5′
-CAA GTA AAT GCA GAA ACA GG-3′
(SEQ ID NO;
4). - View Dependent Claims (2, 3)
- -TGA GAA GGC AGT AGA AAG CTT AG-3′
Specification