DIFFERENTIAL EXPRESSION GENE PROFILES AND APPLICATIONS IN MOLECULAR STAGING OF HUMAN GASTRIC CANCER
First Claim
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1. A method for diagnosing intestinal gastric cancer in a mammal, comprising:
- (a) detecting levels of expression of at least two genes shown in FIG. 1 in an intestinal gastric tissue of the mammal, wherein the tissue is suspected of being cancerous;
(b) detecting levels of expression of the genes in an intestinal gastric tissue adjacent to the tissue suspected of being cancerous of the mammal; and
(c) comparing the levels of expressions of the genes in the tissue suspected of being cancerous to the levels of expression in the adjacent tissue;
wherein substantial variance of the levels of expression of the genes between the tissue suspected of being cancerous and the adjacent tissue is indicative of presence of intestinal gastric cancer in the mammal.
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Abstract
The invention provides methods for detecting differential gene expression in intestinal gastric tissue in a mammal by comparing the expression of specific genes in an intestinal gastric tissue suspected of being cancerous with that of the corresponding adjacent intestinal gastric tissue or a normal gastric mucosa tissue. The methods can be used in diagnosing or monitoring the progression of intestinal gastric cancer and determining the levels of differentiation of intestinal gastric cancer Systems and kits for methods of the invention are also provided.
19 Citations
36 Claims
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1. A method for diagnosing intestinal gastric cancer in a mammal, comprising:
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(a) detecting levels of expression of at least two genes shown in FIG. 1 in an intestinal gastric tissue of the mammal, wherein the tissue is suspected of being cancerous;(b) detecting levels of expression of the genes in an intestinal gastric tissue adjacent to the tissue suspected of being cancerous of the mammal; and (c) comparing the levels of expressions of the genes in the tissue suspected of being cancerous to the levels of expression in the adjacent tissue;
wherein substantial variance of the levels of expression of the genes between the tissue suspected of being cancerous and the adjacent tissue is indicative of presence of intestinal gastric cancer in the mammal. - View Dependent Claims (2, 3, 4, 5)
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6. A system for diagnosing intestinal gastric cancer in a mammal, consisting essentially of a plurality of isolated polynucleotide molecules, wherein each isolated polynucleotide molecule is capable of detecting expression of a gene selected from the group consisting of at least 61 genes shown in
FIG. 1 , whereby differential expression of said genes can be detected.
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10. A method for diagnosing intestinal gastric cancer in a mammal, comprising:
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(a) detecting levels of expression of at least two genes shown in FIG. 2 in an intestinal gastric tissue suspected of being cancerous of the mammal;(b) detecting levels of expression of the genes in an intestinal gastric tissue adjacent to the gastric tissue suspected of being cancerous; (c) comparing the levels of expression of the genes in the gastric tissue suspected of being cancerous to levels of expression of the genes in a normal intestinal gastric mucosa tissue of the same mammal species; (d) comparing the levels of expression of the genes in the adjacent gastric tissue to levels of expression of the genes in a normal intestinal gastric mucosa tissue of the same mammal species; and (e) comparing the levels of expression of the genes in the gastric tissue suspected of being cancerous to the levels of expression of the genes in the adjacent gastric tissue; wherein substantial variance of the levels of expression of the genes shown in FIG. 2 between the gastric tissue suspected of being cancerous and the normal gastric mucosa tissue and between the adjacent gastric tissue and the normal gastric mucosa tissue, and no substantial variance of the levels of expression of the genes shown inFIG. 2 between the gastric tissue suspected of being cancerous and the adjacent gastric tissue is indicative of presence of intestinal gastric cancer in the mammal. - View Dependent Claims (11, 12, 13, 14)
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15. A system for diagnosing intestinal gastric cancer in a mammal, consisting essentially of a plurality of isolated polynucleotide molecules, wherein each isolated polynucleotide molecule is capable of detecting expression of a gene selected from the group consisting of at least 50 genes shown in
FIG. 2 , whereby differential expression of said genes can be detected.
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19. A method for assessing levels of differentiation of intestinal gastric cancer in a mammal, comprising:
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(a) detecting levels of expression of at least two genes shown in FIG. 3 in an intestinal gastric cancer tissue of the mammal;(b) detecting levels of expression of the genes in an intestinal gastric tissue adjacent to the cancer tissue; and (c) comparing the levels of expression of the genes in the cancer tissue to the levels of expression of the genes in the adjacent tissue; wherein substantial variance of the levels of expression of the genes between the cancer tissue and the adjacent tissue is indicative of high level of differentiation of the intestinal gastric cancer in the mammal. - View Dependent Claims (20, 21, 22, 23)
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24. A system for assessing levels of differentiation of intestinal gastric cancer in a mammal, consisting essentially of a plurality of isolated polynucleotide molecules, wherein each isolated polynucleotide molecule is capable of detecting expression of a gene selected from the group consisting of at least 33 genes shown in
FIG. 3 , whereby differential expression of said genes can be detected.
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28. A method for assessing levels of differentiation of intestinal gastric cancer in a mammal, comprising:
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(a) detecting levels of expression of at least two genes shown in FIG. 4 in an intestinal gastric cancer tissue of the mammal;(b) detecting levels of expression of the genes in an intestinal gastric tissue adjacent to the cancer tissue; and (c) comparing the levels of expression of the genes in the cancer tissue to the levels of expression in the adjacent tissue; wherein substantial variance of the levels of expression of the genes between the cancer tissue and the adjacent tissue is indicative of low level of differentiation of the intestinal gastric cancer in the mammal. - View Dependent Claims (29, 30, 31, 32)
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33. A system for assessing levels of differentiation of intestinal gastric cancer in a mammal, consisting essentially of a plurality of isolated polynucleotide molecules, wherein each isolated polynucleotide molecule is capable of detecting expression of a gene selected from the group consisting of at least 27 genes shown in
FIG. 4 , whereby differential expression of said genes can be detected.
Specification