IMPEDANCE SPECTROSCOPY OF BIOMOLECULES USING FUNCTIONALIZED NANOPARTICLES
First Claim
1. A method of detecting and analyzing biomolecules in a sample analyte, the method comprising:
- functionalizing a plurality of metallic nanoparticles;
providing a plurality of electrodes, each electrode having an exposed surface;
applying the plurality of functionalized metallic nanoparticles and the sample analyte to the plurality of electrodes; and
using impedance spectroscopy to detect a sample signal profile for a group of sample electrical parameters across a selected frequency range.
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Accused Products
Abstract
A biosensor system includes a functionalized interdigitated electrode, functionalized nanoparticles, a current/voltage signal generator, and a circuit analyzer. The interdigitated electrode can be functionalized by coating an exposed surface with first biomolecular probes. The nanoparticles are functionalized by coating an outer surface with second biomolecular probes. A signal generator provides a signal (e.g., an alternating current or voltage) having a selected range of frequencies. A circuit analyzer analyzes electrical parameters of the circuit as the signal is applied. Sensitivity is increased by the presence of functionalized nanoparticles in the system. An analytic method includes measuring changes in electrical parameters of the circuit over the range of frequencies. Using these measurements, the biosensor system can determine whether a target biomolecule is bound. The biosensor system can also identify a biomolecule by comparing the detected signal or “electro-fingerprint” with a reference set of signals over the frequency range.
48 Citations
32 Claims
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1. A method of detecting and analyzing biomolecules in a sample analyte, the method comprising:
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functionalizing a plurality of metallic nanoparticles; providing a plurality of electrodes, each electrode having an exposed surface; applying the plurality of functionalized metallic nanoparticles and the sample analyte to the plurality of electrodes; and using impedance spectroscopy to detect a sample signal profile for a group of sample electrical parameters across a selected frequency range. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A biosensor system for detecting or identifying biomolecules in a sample analyte, the biosensor system comprising:
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a substrate; an electrode formed on the substrate, the electrode including one or more pairs of opposed fingers, each finger having an exposed upper surface and exposed side walls; a stimulator electrically coupled to the electrode and structured to provide a plurality of input frequencies over a selected frequency range; a detector operative to detect a signal of the sample analyte over the selected frequency range and generate a sample signal profile for a group of sample electrical parameters; means for comparing the sample signal profile with a reference signal profile across the selected frequency range; and a plurality of functionalized nanoparticles. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26, 27)
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28. A method of detecting and analyzing biomolecules in a reference sample analyte, the method comprising:
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functionalizing a plurality of metallic nanoparticles; providing a plurality of electrodes, each electrode having an exposed surface; applying the plurality of functionalized metallic nanoparticles and the reference sample analyte to the plurality of electrodes; and using impedance spectroscopy to detect a reference sample signal profile for a group of sample electrical parameters across a selected frequency range. - View Dependent Claims (29, 30, 31, 32)
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Specification