MODIFIED GREEN FLUORESCENT PROTEINS AND METHODS FOR USING SAME

• US 20090117650A1
• Filed: 07/24/2008
• Published: 05/07/2009
• Est. Priority Date: 11/04/2005
• Status: Active Grant

• Current Assignee: Evrogen Joint Stock Company
• Original Assignee: Evrogen Joint Stock Company
• Inventors: Lukyanov, Sergey A.
• Granted Patent: US 7,888,113 B2
• Time in Patent Office: Days
• US Class Current: 435/325
• CPC Class Codes: C07K 14/43595 from coelenteratae, e.g. medusae

Abstract

The present invention provides nucleic acid molecules encoding mutant fluorescent proteins as well as proteins encoded by these nucleic acids. In addition, host-cells, stable cell lines and transgenic organisms comprising the above-referenced nucleic acid molecules are provided. The subject protein and nucleic acid compositions find use in a variety of different applications and methods, particularly for labeling of biomolecules, cells, or cell organelles.

Claims

1. An isolated nucleic acid, comprising a nucleic acid sequence encoding a genetically engineered fluorescent protein that has at least 95% identity with the amino acid sequence selected from the group consisting of SEQ ID NO:

22, 24.

2. An isolated nucleic acid of claim 1, wherein the genetically engineered fluorescent protein that has at least 97% identity with the amino acid sequence selected from the group consisting of SEQ ID NO:

22, 24.

3. The nucleic acid of claim 1, wherein the genetically engineered fluorescent protein has the amino acid sequence selected from the group consisting of SEQ ID NO:

22, 24.

4. A vector comprising the nucleic acid of claim 1.

5. An expression cassette comprising:

(a) a transcriptional initiation region that is functional in an expression host;

(b) the nucleic acid according to claim 1; and

(c) and a transcriptional termination region functional in said expression host.

6. An host cell or progeny thereof, comprising the expression cassette according to claim 5 as part of an extrachromosomal element or integrated into the genome of a host cell as a result of introduction of said expression cassette into said host cell.

7. A transgenic cell, or progeny thereof, comprising the nucleic acid according to claim 1.

8. A kit comprising at least one nucleic acid according to claim 1.