Cellular upregulation of VEGF receptor expression using nanofibrillar articles
First Claim
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1. A method for selectively up-regulating the expression of a VEGF receptor in a cell comprising a step of:
- contacting a cell comprising a VEGF receptor gene with a synthetic nanofibrillar article for a period of time wherein expression of the VEGF receptor gene becomes selectively upregulated in the cell.
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Abstract
The invention provides methods and systems for selectively upregulating the expression of a VEGF receptor in a cell using a synthetic nanofibrillar article. Exemplary embodiments relate to the upregulation of the VEGF receptor Flk-1 in endothelial cells. Cells having the selectively-upregulated VEGF receptor can be used in processes such as cell culturing, tissue engineering, cellular therapy, and drug discovery.
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22 Claims
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1. A method for selectively up-regulating the expression of a VEGF receptor in a cell comprising a step of:
contacting a cell comprising a VEGF receptor gene with a synthetic nanofibrillar article for a period of time wherein expression of the VEGF receptor gene becomes selectively upregulated in the cell. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method for treating a medical condition in a subject comprising steps of:
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providing cells comprising a VEGF receptor gene, contacting the cells with an synthetic nanofibrillar article for a period of time wherein expression of the VEGF receptor gene becomes selectively upregulated in the cells, and implanting the cells in which the VEGF receptor gene has become selectively upregulated in a subject to treat a medical condition. - View Dependent Claims (16, 17, 18, 19)
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20. A method for identifying a compound that affects or interacts with cells wherein a VEGF receptor has been selectively upregulated, comprising steps of:
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providing cells comprising a VEGF receptor, contacting the cells with a synthetic nanofibrillar article for a period of time wherein expression of the VEGF receptor gene becomes selectively upregulated in the cells, applying a compound to the cells in which expression of the VEGF receptor gene is selectively upregulated, and determining if the compound affects or interacts with cells in which expression of the VEGF receptor gene is selectively upregulated as compared to a control. - View Dependent Claims (21, 22)
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Specification