Biodegradable Colloidal Gels as Moldable Tissue Engineering Scaffolds

• US 20090123509A1
• Filed: 11/05/2008
• Published: 05/14/2009
• Est. Priority Date: 11/08/2007
• Status: Abandoned Application

• Current Assignee: University of Kansas
• Original Assignee: University of Kansas
• Inventors: Wang, Qun, Berkland, Cory
• Application Number:
  US12/265,634
• Publication Number:
  US 20090123509A1
• Time in Patent Office: Days
• US Class Current: 424/422
• CPC Class Codes: A61L 26/0057 Ingredients of undetermined constitution or reaction products thereof
• CPC Class Codes: A61L 26/008 Hydrogels or hydrocolloids
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Abstract

A colloid gel can include a plurality of positive charged particles mixed and associated with a plurality of negative charged particles so as to form a three-dimensional matrix having a plurality of pores defined by and disposed between the particles. The three-dimensional matrix can have shear thinning under shear and structure stability in the absence of shear. A method of manufacturing the colloid gel can include combining the positive charged particles with the negative charged particles, in a mold or in situ, so as to form the three-dimensional matrix having the plurality of pores.

Claims

1. A biocompatible colloid gel comprising:

a plurality of positive charged biocompatible particles; and

a plurality of negative charged biocompatible particles associated with the plurality of positive charged particles so as to form a three-dimensional matrix having a plurality of pores defined by and disposed between the particles, said three-dimensional matrix having shear thinning under shear and structure stability in the absence of shear.

2. A colloid gel as in claim 1, wherein at least a portion of the plurality of positive charged particles and plurality of negatively charged particles are nanoparticles.

3. A colloid gel as in claim 1, wherein a majority of the plurality of positive charged particles and plurality of negatively charged particles are nanoparticles.

4. A colloid gel as in claim 1, wherein one of the plurality of positive charged particles or plurality of negative charged particles is a plurality of polymer molecules having the opposite charge of the other plurality of particles.

5. A colloid gel as in claim 1, wherein the colloid gel is disposed in a syringe.

6. A colloid gel as in claim 1, wherein the colloid gel is disposed within a subject.

7. A colloid gel as in claim 1, wherein the colloid gel is topically disposed in or on a wound of a subject.

8. A colloid gel as in claim 1, further comprising at least one bioactive agent disposed within the three-dimensional matrix.

9. A colloid gel as in claim 8, wherein the bioactive agent is disposed within at least one particle and/or within an interstitial space between the particles.

10. A colloid gel as in claim 1, further comprising cells disposed and growing within the pores.

12. A method as in claim 10, further comprising preparing a majority of the plurality of positive charged particles and plurality of negatively charged particles as nanoparticles.

13. A method as in claim 12, wherein one of the plurality of positive charged particles or plurality of negative charged particles is a plurality of polymer molecules having the opposite charge of the other plurality of particles.

14. A method as in claim 10, further comprising introducing the colloid gel into a syringe.

15. A method as in claim 10, further comprising introducing the colloid gel into a subject as an implant.

16. A method as in claim 10, wherein the positive charged particles are adjacent and ionically associated with the negative charged particles so as to form the three-dimensional matrix and pores.

17. A method as in claim 1, further comprising introducing the colloid gel into or onto a wound of a subject.

18. A method as in claim 10, further comprising introducing at least one bioactive agent into the three-dimensional matrix.

19. A method as in claim 18, further comprising introducing the bioactive agent into at least one particle and/or an interstitial space between the particles.

20. A method as in claim 10, further comprising introducing cells into the pores.

11. A method for manufacturing a biocompatible colloid gel, the method comprising:

providing a plurality of positive charged biocompatible particles;

providing a plurality of negative charged biocompatible particles; and

combining the positive charged particles with the negative charged particles so as to form a three-dimensional matrix having a plurality of pores defined by and disposed between the positive and negative charged particles, said three-dimensional matrix having shear thinning under shear and structure stability in the absence of shear.

21. A method of forming an implant in situ, the method comprising:

providing a colloid gel formed by combining positive charged particles with negative charged particles so as to form a three-dimensional matrix having a plurality of pores defined by and disposed between the positive and negative charged particles, said three-dimensional matrix having shear thinning under shear and structure stability in the absence of shear; and

injecting the colloid gel into a subject so as to form an implant.

22. A method as in claim 21, further comprising:

preparing a majority of the plurality of positive charged particles and plurality of negatively charged particles as nanoparticles; and

combining the positive charged particles and plurality of negatively charged particles to form the colloid gel.

23. A method as in claim 22, wherein one of the plurality of positive charged particles or plurality of negative charged particles is a plurality of polymer molecules having the opposite charge of the other plurality of particles.

24. A method as in claim 21, further comprising introducing the colloid gel into a syringe.

25. A method as in claim 21, further comprising shaping the colloid gel into a shape of the implant while within the subject.

26. A method as in claim 21, wherein the positive charged particles are adjacent and ionically associated with the negative charged particles so as to form the three-dimensional matrix and pores.

27. A method as in claim 21, further comprising introducing at least one bioactive agent into the three-dimensional matrix prior to the injecting.

28. A method as in claim 27, further comprising introducing the bioactive agent into at least one particle.

29. A method as in claim 27, further comprising introducing the bioactive agent into an interstitial space between the particles.

30. A biocompatible colloid gel for use in tissue engineering comprising:

a plurality of charged biocompatible particles having a first charge; and

a plurality of charged biocompatible polymers having a charge opposite of the first charge associated with the plurality of charged particles having the first charge so as to form a three-dimensional matrix, said three-dimensional matrix having shear thinning under shear and structure stability in the absence of shear.