Genomic Assay
First Claim
1. A method of detecting a nucleic acid sequence in a genomic sample, said method comprising:
- providing the genomic sample comprising a target nucleic acid sequence of a duplex nucleic acid;
providing a probe comprising a probe nucleic acid sequence;
providing a hybridization mixture comprising the genomic sample, the probe, a hybridization promoting agent and labels;
incubating the hybridization mixture to provide an incubated mixture comprising a complex of the target nucleic acid sequence, the probe and the labels;
irradiating the incubated mixture with radiation effective to stimulate at least some of the labels to emit energy; and
detecting from a fluorescent signal whether the probe perfectly matches the target nucleic acid sequence, to thereby detect whether the nucleic acid sequence is present in the genomic sample,wherein the detecting is completed within sixty minutes of providing the hybridization mixture, and the method is conducted without denaturing the duplex nucleic acid and without PCR amplification of the duplex nucleic acid.
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Accused Products
Abstract
A method of detecting a nucleic acid sequence in a genomic sample, includes: providing the genomic sample containing a target nucleic acid sequence of a duplex nucleic acid; providing, a probe containing a probe nucleic acid sequence; providing a hybridization mixture containing the genomic sample, the probe, a hybridization promoting agent and labels; incubating the hybridization mixture; irradiating the incubated mixture with radiation effective to stimulate at least some of the labels to emit energy; and detecting from a fluorescent signal whether the probe perfectly matches the target nucleic acid sequence, wherein the detecting is completed within sixty minutes of the hybridization mixture providing, and the method is conducted without denaturing and without PCR amplifying the duplex nucleic acid. A kit for practicing the method includes the probe, the hybridization promoting agent, and labels.
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Citations
69 Claims
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1. A method of detecting a nucleic acid sequence in a genomic sample, said method comprising:
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providing the genomic sample comprising a target nucleic acid sequence of a duplex nucleic acid; providing a probe comprising a probe nucleic acid sequence; providing a hybridization mixture comprising the genomic sample, the probe, a hybridization promoting agent and labels; incubating the hybridization mixture to provide an incubated mixture comprising a complex of the target nucleic acid sequence, the probe and the labels; irradiating the incubated mixture with radiation effective to stimulate at least some of the labels to emit energy; and detecting from a fluorescent signal whether the probe perfectly matches the target nucleic acid sequence, to thereby detect whether the nucleic acid sequence is present in the genomic sample, wherein the detecting is completed within sixty minutes of providing the hybridization mixture, and the method is conducted without denaturing the duplex nucleic acid and without PCR amplification of the duplex nucleic acid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 63, 64, 65, 66, 67, 68, 69)
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61. A method of detecting a nucleic acid sequence in a genomic sample, said method comprising:
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providing the genomic sample comprising a target nucleic acid sequence of a single-stranded or double-stranded nucleic acid; providing a probe comprising a probe nucleic acid sequence; providing a hybridization mixture comprising the genomic sample, the probe, a hybridization promoting agent and labels; incubating the hybridization mixture to provide an incubated mixture comprising a complex of the target nucleic acid sequence, the probe and the labels; applying energy to the incubated mixture effective to elicit a signal from the hybridization mixture; and detecting from the signal whether the probe perfectly matches the target nucleic acid sequence, to thereby detect whether the nucleic acid sequence is present in the genomic sample, wherein the detecting is completed within sixty minutes of providing of the hybridization mixture, and the method is conducted without denaturing the duplex nucleic acid and without PCR amplification of the duplex nucleic acid. - View Dependent Claims (62)
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Specification