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Genomic Assay

  • US 20090123914A1
  • Filed: 09/23/2005
  • Published: 05/14/2009
  • Est. Priority Date: 09/24/2004
  • Status: Active Grant
First Claim
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1. A method of detecting a nucleic acid sequence in a genomic sample, said method comprising:

  • providing the genomic sample comprising a target nucleic acid sequence of a duplex nucleic acid;

    providing a probe comprising a probe nucleic acid sequence;

    providing a hybridization mixture comprising the genomic sample, the probe, a hybridization promoting agent and labels;

    incubating the hybridization mixture to provide an incubated mixture comprising a complex of the target nucleic acid sequence, the probe and the labels;

    irradiating the incubated mixture with radiation effective to stimulate at least some of the labels to emit energy; and

    detecting from a fluorescent signal whether the probe perfectly matches the target nucleic acid sequence, to thereby detect whether the nucleic acid sequence is present in the genomic sample,wherein the detecting is completed within sixty minutes of providing the hybridization mixture, and the method is conducted without denaturing the duplex nucleic acid and without PCR amplification of the duplex nucleic acid.

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