Bead Bound Combinatorial Oligonucleoside Phosphorothioate And Phosphorodithioate Aptamer Libraries
0 Assignments
0 Petitions
Accused Products
Abstract
The present invention includes composition and methods for making and using a combinatorial library having two or more beads, wherein attached to each bead is a unique nucleic acid aptamer that have disposed thereon a unique sequence. The library aptamers may be attached covalently to the one or more beads, which may be polystyrene beads. The aptamers may include phosphorothioate, phosphorodithioate and/or methylphosphonate linkages and may be single or double stranded DNA, RNA or even PNAs.
76 Citations
108 Claims
-
1-81. -81. (canceled)
-
82. A method for isolation of a target comprising the steps of:
-
dispersing one or more probe beads comprising an aptamer library in a thixotropic agent; contacting the probe beads with a target; scanning for probe beads that generate a detectable signal from interaction between the one or more probe beads and the target; and picking one or more probe beads based on the detectable signal. - View Dependent Claims (83, 84, 85, 86, 87, 88, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108)
-
Specification