Multipotent adult stem cells
First Claim
1. An isolated human multipotent adult stem cell selected from the group consisting of:
- an isolated human hair-follicle derived multipotent adult stem cell having one or more characteristics selected from the group consisting of;
expresses one or more of;
nanog, Oct4 and Nestin;
can differentiate in culture into one or more of the following;
a neuronal cell, a glial cell, a melanocytic cell, a muscle cell, an adipocyte, an osteocyte, a chondrocyte, an islet cell, and a lymphocyte; and
grows in cell culture in spheres; and
an isolated human multipotent adult stem cell selected from the group consisting of;
an isolated human multipotent adult stem cell that expresses nanog;
an isolated human multipotent adult stem cell that expresses Oct4 and is free of stage specific embryonic antigen-3 and stage specific embryonic antigen-4; and
an isolated human multipotent adult stem cell that expresses nanog and Oct4 and is free of stage specific embryonic antigen-3 and stage specific embryonic antigen-4.
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Accused Products
Abstract
Isolated human multipotent adult stem cell and isolated populations of cells that include human multipotent adult stem cells are disclosed. Human hair-follicle derived multipotent adult stem cells and methods of preparing isolated populations of cells that include human multipotent adult stem cells are disclosed. Isolated human hair-follicle derived multipotent adult stem cell that can differentiate in culture into a neuronal cell, a glial cell, a melanocyte cell, a muscle cell, an osteocyte, a chondrocyte, and a lymphocyte. Isolated human hair-follicle derived multipotent adult stem cell that can grow in cell culture in spheres are disclosed. Human pancreas derived multipotent adult stem cells, human liver derived multipotent adult stem cells, human kidney derived multipotent adult stem cells, human heart derived multipotent adult stem cells, human neural derived multipotent adult stem cells and methods of preparing isolated populations of cells that include such human multipotent adult stem cells are disclosed. Method of treating an individual who has diabetes, cardiac muscle damage, muscle damage and disease, neurodegenerative disease or nerve damage or injury, bone loss, damage and/or disease, cartilage loss, damage and/or disease, hair loss and immune disorders, are disclosed.
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Citations
45 Claims
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1. An isolated human multipotent adult stem cell selected from the group consisting of:
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an isolated human hair-follicle derived multipotent adult stem cell having one or more characteristics selected from the group consisting of; expresses one or more of;
nanog, Oct4 and Nestin;can differentiate in culture into one or more of the following;
a neuronal cell, a glial cell, a melanocytic cell, a muscle cell, an adipocyte, an osteocyte, a chondrocyte, an islet cell, and a lymphocyte; andgrows in cell culture in spheres; and an isolated human multipotent adult stem cell selected from the group consisting of; an isolated human multipotent adult stem cell that expresses nanog; an isolated human multipotent adult stem cell that expresses Oct4 and is free of stage specific embryonic antigen-3 and stage specific embryonic antigen-4; and an isolated human multipotent adult stem cell that expresses nanog and Oct4 and is free of stage specific embryonic antigen-3 and stage specific embryonic antigen-4. - View Dependent Claims (2, 3, 4)
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5-7. -7. (canceled)
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8. An isolated population of human multipotent adult stem cells selected from the group consisting of:
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a) human multipotent adult stem cells that express nanog and is free of stage specific embryonic antigen-3 and stage specific embryonic antigen-4; human multipotent adult stem cells that express Oct4 and is free of stage specific embryonic antigen-3 and stage specific embryonic antigen-4; human multipotent adult stem cells that express Nestin and is free of stage specific embryonic antigen-3 and stage specific embryonic antigen-4; human multipotent adult stem cells that express nanog and Oct4 and is free of stage specific embryonic antigen-3 and stage specific embryonic antigen-4; and combinations thereof; b) human hair-follicle derived multipotent adult stem cells that express nanog; human hair follicle-derived multipotent adult stem cells that express Oct4; human hair follicle-derived multipotent adult stem cells that express Nestin; human hair-follicle derived multipotent adult stem cells that express nanog and Oct4; and combinations thereof; c) human hair-follicle derived multipotent adult stem cells that can differentiate in culture into a neuronal cell, a glial cell, a melanocytic cell, a muscle cell, an adipocyte, an osteocyte, a chondrocyte, an islet cell, and a lymphocyte; d) human hair-follicle derived multipotent adult stem cell that grows in cell culture in spheres; and e) isolated cells formed by the method of forming a single cell suspension of cells disassociated from a sample that comprises cells from an individual, and culturing such cells in conditions suitable for culturing and maintaining human embryonic stem cells or in conditions comprising a serum free minimal medium supplemented with human basic fibroblast growth factor and human epidermal growth factor;
wherein multipotent adult stem cells are identified and isolated from said culture. - View Dependent Claims (10, 42, 43)
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9. (canceled)
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11-12. -12. (canceled)
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13. A method of preparing an isolated population of human hair-follicle derived multipotent adult stem cells comprising the steps of:
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forming a single cell suspension of cells disassociated from follicular epithelium of a hair shaft and culturing such cells in conditions suitable for culturing and maintaining human embryonic stem cells wherein spheres form that comprise human hair-follicle derived multipotent adult stem cells or in conditions comprising a serum free minimal medium supplemented with human basic fibroblast growth factor and human epidermal growth factor;
orculturing a human hair follicle in conditions suitable for culturing and maintaining human embryonic stem cells wherein spheres form that comprise human hair-follicle derived multipotent adult stem cells or in conditions comprising a serum free minimal medium supplemented with human basic fibroblast growth factor and human epidermal growth factor, wherein said hair follicle is cultured under such conditions for a time sufficient for cells to grow on said hair follicle, and isolating from said cells a population of human hair-follicle derived multipotent adult stem cells.
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14-22. -22. (canceled)
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23. A method of treating an individual comprising the steps selected from the group consisting of:
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a) administering to said individual human isolated human hair-follicle derived multipotent adult stem cells having one or more characteristics selected from the group consisting of; expresses one or more of;
nanog, Oct4 and Nestin;can differentiate in culture into one or more of the following;
a neuronal cell, a glial cell, a melanocytic cell, a muscle cell, an adipocyte, an osteocyte, a chondrocyte, an islet cell, and a lymphocyte; andgrows in cell culture in spheres; b) administering to said individual an isolated human multipotent adult stem cells selected from the group consisting of; human multipotent adult stem cells that express nanog; human multipotent adult stem cells that express Oct4 and are free of stage specific embryonic antigen-3 and stage specific embryonic antigen-4; human hair-follicle derived multipotent adult stem cells that express nanog and Oct4 and are free of stage specific embryonic antigen-3 and stage specific embryonic antigen-4; and human hair-follicle derived multipotent adult stem cells that can differentiate in culture into a neuronal cell, a glial cell, a melanocytic cell, a muscle cell, an adipocyte, an osteocyte, a chondrocyte, an islet cell, and a lymphocyte; and human hair-follicle derived multipotent adult stem cell that grows in cell culture in spheres; and c) administering to said individual human isolated population of multipotent adult stem cells having cells selected from the group consisting of; human multipotent adult stem cells that express nanog and are free of stage specific embryonic antigen-3 and stage specific embryonic antigen-4; human multipotent adult stem cells that express Oct4 and are free of stage specific embryonic antigen-3 and stage specific embryonic antigen-4; human multipotent adult stem cells that express Nestin and are free of stage specific embryonic antigen-3 and stage specific embryonic antigen-4; human multipotent adult stem cells that express nanog and Oct4 and are free of stage specific embryonic antigen-3 and stage specific embryonic antigen-combinations thereof, d) administering to said individual human isolated population of human hair-follicle derived multipotent adult stem cells having cells selected from the group consisting of; human hair-follicle derived multipotent adult stem cells that express nanog; human hair follicle-derived multipotent adult stem cells that express Oct4; human hair follicle-derived multipotent adult stem cells that express Nestin; human hair-follicle derived multipotent adult stem cells that express nanog and Oct4; and combinations thereof; e) administering to said individual human isolated population of human hair-follicle derived multipotent adult stem cells having cells can differentiate in culture into one or more of the following;
a neuronal cell, a glial cell, a melanocytic cell, a muscle cell, an adipocyte, an osteocyte, a chondrocyte, an islet cell, and a lymphocyte; andf) administering to said individual human isolated population of human hair-follicle derived multipotent adult stem cells having cells that grow in cell culture in spheres; g) administering to said individual human isolated population of human hair-follicle derived multipotent adult stem cells formed by the method of forming a single cell suspension of cells disassociated from a sample that comprises cells from an individual and culturing such cells in conditions suitable for culturing and maintaining human embryonic stem cells or in conditions comprising a serum free minimal medium supplemented with human basic fibroblast growth factor and human epidermal growth factor;
wherein multipotent adult stem cells are identified and isolated from said culture and wherein said sample consists of cells from the follicular epithelium of a hair shaft a sample of liver tissue a sample of pancreatic tissue a sample of cardiac tissue and a sample of nerve cells.and h) administering to said individual isolated cells isolated by preparing an isolated population of human multipotent adult stem cells by forming a single cell suspension of cells disassociated from sample that comprises cells from an individual, and culturing such cells in conditions suitable for culturing and maintaining human embryonic stem cells or in conditions comprising a serum free minimal medium supplemented with human basic fibroblast growth factor and human epidermal growth factor;
wherein multipotent adult stem cells are identified and isolated from said culture. - View Dependent Claims (25, 44, 45)
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24. (canceled)
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26. A method of preparing an isolated population of human multipotent adult stem cells comprising the steps of:
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a) forming a single cell suspension of cells disassociated from sample that comprises cells from an individual, and b) culturing such cells in conditions suitable for culturing and maintaining human embryonic stem cells or in conditions comprising a serum free minimal medium supplemented with human basic fibroblast growth factor and human epidermal growth factor;
wherein multipotent adult stem cells are identified and isolated from said culture. - View Dependent Claims (30, 32)
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27-29. -29. (canceled)
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31. (canceled)
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33-41. -41. (canceled)
Specification