Methods of Detection of Changes in Cells
First Claim
Patent Images
1. A method of detection of responses of cells to stimuli comprising:
- (a) (i) immobilizing one or more extracellular matrix ligands to a surface of a colorimetric resonant reflectance biosensor or a grating-based waveguide biosensor, wherein the cells have cell surface receptors specific for the one or more extracellular matrix ligands; and
adding the cells to the biosensor;
or(ii) mixing cells with one or more extracellular matrix ligands, wherein the cells have cell surface receptors specific for the one or more extracellular matrix ligands; and
adding the cells with one or more extracellular matrix ligands to a surface of a colorimetric resonant reflectance biosensor or a grating-based waveguide biosensor;
(b) exposing the cells to stimuli; and
(d) detecting the response of the cells to the stimuli.
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Abstract
Methods are provided to detect changes in cells without the use of detection labels.
123 Citations
27 Claims
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1. A method of detection of responses of cells to stimuli comprising:
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(a) (i) immobilizing one or more extracellular matrix ligands to a surface of a colorimetric resonant reflectance biosensor or a grating-based waveguide biosensor, wherein the cells have cell surface receptors specific for the one or more extracellular matrix ligands; and
adding the cells to the biosensor;
or(ii) mixing cells with one or more extracellular matrix ligands, wherein the cells have cell surface receptors specific for the one or more extracellular matrix ligands; and
adding the cells with one or more extracellular matrix ligands to a surface of a colorimetric resonant reflectance biosensor or a grating-based waveguide biosensor;(b) exposing the cells to stimuli; and (d) detecting the response of the cells to the stimuli. - View Dependent Claims (2, 3, 4)
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9. A method of detection of responses of cells to stimuli comprising:
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(a) washing the cells with buffered saline solution; (b) (i) immobilizing one or more extracellular matrix ligands to a surface of a colorimetric resonant reflectance biosensor or a grating-based waveguide biosensor, wherein the cells have cell surface receptors specific for the one or more extracellular matrix ligands; and
adding the cells to the biosensor;
or(ii) mixing cells with one or more extracellular matrix ligands, wherein the cells have cell surface receptors specific for the one or more extracellular matrix ligands; and
adding the cells with one or more extracellular matrix ligands to a surface of a colorimetric resonant reflectance biosensor or a grating-based waveguide biosensor;(c) exposing the cells to stimuli; and (d) detecting the response of the cells to the stimuli. - View Dependent Claims (10, 11, 12)
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23. A method of identifying a compound that affects adhesion of cells comprising:
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(a) (i) immobilizing one or more extracellular matrix ligands to a surface of a colorimetric resonant reflectance biosensor or a grating-based waveguide biosensor, wherein the cells have cell surface receptors specific for the one or more extracellular matrix ligands; and
adding the cells to the biosensor;
or(ii) mixing cells with one or more extracellular matrix ligands, wherein the cells have cell surface receptors specific for the one or more extracellular matrix ligands; and
adding the cells with one or more extracellular matrix ligands to a surface of a colorimetric resonant reflectance biosensor or a grating-based waveguide biosensor;(b) treating the cells with a test compound; (c) detecting a colorimetric resonant reflectance optical first PWV or signal and comparing the first PWV or signal to a second PWV or signal from control cells that were not treated with the test compound to determine if the test compound affects adhesion of the cells. - View Dependent Claims (24, 25)
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Specification