Novel fungal proteins and nucleic acids encoding same

• US 20090130737A1
• Filed: 07/03/2008
• Published: 05/21/2009
• Est. Priority Date: 08/25/2003
• Status: Active Grant

• Current Assignee: AMYRA Biotech AG
• Original Assignee: Funzyme Biotechnologies SA
• Inventors: Grouzmann, Eric, Stocklin, Reto, Monod, Michel
• Granted Patent: US 7,943,340 B2
• Time in Patent Office: Days
• US Class Current: 435/223
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61P 1/00 Drugs for disorders of the alimentary tract or the digestive system
• CPC Class Codes: A61P 1/14 Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
• CPC Class Codes: A61P 31/10 Antimycotics
• CPC Class Codes: A61P 37/08 Antiallergic agents antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C07K 14/37 from fungi
• CPC Class Codes: C12N 9/48 acting on peptide bonds (3.4)
• CPC Class Codes: C12P 21/06 produced by the hydrolysis of a peptide bond, e.g. hydrolysate products preparing foodstuffs by protein hydrolysis A23J3/00
• CPC Class Codes: C12Q 1/18 Testing for antimicrobial activity of a material
• CPC Class Codes: C12Q 1/37 involving peptidase or proteinase
• CPC Class Codes: G01N 2333/37 from fungi

Abstract

Disclosed herein are fungal nucleic acid sequences that encode novel polypeptides. Also disclosed are polypeptides encoded by these nucleic acid sequences, as well as derivatives, variants, mutants, or fragments of the aforementioned polypeptide, polynucleotide, or antibody. The novel leucine aminopeptidase (LAP) and other amino- and carboxypeptidases polypeptides, referred to herein as EXOX nucleic acids and proteins of the invention are useful in a variety of medical, research, and commercial applications.

Claims

1. A method for recovering a protein in its mature form without a di-peptide tag, comprising the steps of:

(1) purification by covalent capture and,

(2) removal of the di-peptide tag with a composition comprising a dipeptidyl peptidase IV (DPPIV) of SEQ ID NO;

35, a biologically active fragment thereof, a naturally occurring allelic variants thereof, or a sequence having at least 90% of identity.

2. The method of claim 1, wherein the di-peptide tag comprises an Xaa-Pro tag.

3. The method of claim 2, wherein said Xaa is an amino acid comprising at least two vicinal nucleophilic groups.

4. The method of claim 3, wherein said amino acid is serine, threonine or cysteine.

5. An isolated polypeptide comprising an amino acid sequence consisting of SEQ ID NO:

35.