Automated biological growth and dispensing system

• US 20090130740A1
• Filed: 11/03/2008
• Published: 05/21/2009
• Est. Priority Date: 09/04/2003
• Status: Active Grant

• Current Assignee: Gotohti.Com Inc.
• Original Assignee: Gotohti.Com Inc.
• Inventors: Ophardt, Heiner
• Granted Patent: US 8,206,973 B2
• Time in Patent Office: Days
• US Class Current: 435/252.100
• CPC Class Codes: C12M 23/28 disposable or single use
• CPC Class Codes: C12M 23/38 Caps; Covers; Plugs; Pouring means
• CPC Class Codes: C12M 23/40 Manifolds; Distribution pieces fluid transfer means B01L3/563
• CPC Class Codes: C12M 23/44 Multiple separable units; Modules
• CPC Class Codes: C12M 27/02 Stirrer or mobile mixing elements
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor

Abstract

An automated biological growth and dispensing system utilizing a modular growing tank which is removable for replacement by another growing tank. Mechanisms for the delivery of air, water and/or nutrients are adapted to permit the growing tank to be readily coupled and uncoupled for easy and inexpensive replacement. Mechanisms for agitation may be integral and removable with the growing tank or may be adapted for a quick connection and disconnection with the growing tank. The growing tank may be disposable and each new growing tank may be provided as a sealed container including a starting amount of a biomass and/or nutrient.

Claims

1. A batch process useful for growing bacteria comprising repeating a batch cycle comprising the steps of:

(i) introducing a batch starter population of bacteria and nutrients, and growing the bacteria in fluid from the batch starter population to a utility population within a predetermined interval and thereafter,

(ii) repeating a sub-cycle of;

(a) dispensing a dispensed portion of bacteria while maintaining a remaining portion of bacteria, and

(b) growing bacteria in the remaining portion to a utility population with the addition of additional fluid and/or nutrients to the tank,

the process carried out in an apparatus comprising;

a modular bio-generation tank having a top, a bottom, a side wall extending upwardly from the base, and a tank outlet for flow of fluid from the tank at a height above the bottom,

an agitation mechanism for agitating fluid in the tank comprising an impeller in the tank rotatable about an axis to discharge fluid impinging on the impeller so as to cause flow of the fluid in the tank which raises fluid in the tank to heights within the tank which increase with increased speed of rotation of the impeller,

wherein during the sub-cycle step (b) of growing bacteria, agitating the fluid by rotating the impeller within a range of speeds which maintain the fluid in the tank at a height below a height of the tank outlet, and

wherein in the sub-cycle step (a), dispensing fluid from the tank by rotating the impeller within a range of speeds which raise the fluid in the tank to a height above the height of the tank outlet.

2. A process according to claim 1 wherein the impeller on rotation creates a standing vortex directing fluid radially outwardly into the side wall and up the side wall,

wherein during the sub-cycle step (b) of growing bacteria, rotating the impeller within a range of speeds which maintain the fluid in the vortex within the tank at a height below the height of the tank outlet, and

wherein during the sub-cycle step (a) of dispensing fluid, rotating the impeller within a range of speeds to increase the fluid in the vortex within the tank to a height above the height of the tank outlet.

3. A process according to claim 2 including while rotating the impeller during sub-cycle step (b) to maintain the fluid in the vortex within the tank at a height below the height of the tank outlet, blowing air downwardly from the top of the tank into the tank into contact with the fluid in the standing vortex to provide oxygen from the air to the fluid.

4. A process as claimed in claim 3 including repeating a plurality of said batch cycle, wherein after each batch cycle removing the modular tank used and replacing the modular tank used by a new modular tank.

5. A process as claimed in claim 1 including repeating a plurality of said batch cycle, wherein after each batch cycle removing the modular tank used and replacing the modular tank used by a new modular tank.

6. A process according to claim 1 wherein the side wall of the tank is generally circular in cross-section normal to the axis disposed co-axially about the axis.

7. A process as claimed in claim 6 wherein the axis is generally vertical.

8. A process as claimed in claim 7 wherein the side wall is disposed at a radius about the axis, the radius of the wall increasing as the wall extends upwardly.

9. A process according to claim 8 wherein the impeller on rotation creates a standing vortex directing fluid radially outwardly into the side wall and up the side wall,

wherein during the sub-cycle step (b) of growing bacteria, rotating the impeller within a range of speeds which maintain the fluid in the vortex within the tank at a height below the height of the tank outlet, and

wherein during the sub-cycle step (a) of dispensing fluid, rotating the impeller within a range of speeds to increase the fluid in the vortex within the tank to a height above the height of the tank outlet.

10. A process according to claim 9 including while rotating the impeller during sub-cycle step (b) to maintain the fluid in the vortex within the tank at a height below the height of the tank outlet, blowing air downwardly from the top of the tank into the tank into contact with the fluid in the standing vortex to provide oxygen from the air to the fluid.

11. A process as claimed in claim 1 wherein after each batch cycle and as a step in the next batch cycle, the tank used in the previous batch cycle is removed and a tank for the next batch cycle is coupled in its place such that a batch starter population of bacteria for each batch cycle is in a tank free of bacteria from a previous batch cycle.

12. A process as claimed in claim 11 wherein the tank outlet comprising an overflow outlet for flow of fluid from the tank under gravity when fluid in the tank is at a height above a height of the overflow outlet,

the top of the tank is open upwardly providing a tank inlet opening,

the side wall is generally circular in cross-section and disposed generally coaxially about a generally vertically disposed axis, and

wherein the process is carried out in an apparatus including:

(a) the tank;

(b) a delivery manifold comprising a removable top lid for the tank covering the tank inlet opening and removably coupled to the tank via a first quick connect and disconnect arrangement, the manifold carrying a water inlet for entry of water into the tank, an air inlet for entry of air into the tank, and a nutrient inlet for entry of nutrients into the tank;

(c) a water delivery system for delivery of water via the water inlet into the tank,

(d) an air delivery system for delivery of air via the air inlet into the tank into contact with fluid in the tank,

(e) a nutrient delivery system for delivery of nutrient via the nutrient inlet into the tank,

(f) an agitation mechanism for agitating fluid in the tank comprising an impeller in the tank for rotation about the axis directing fluid impinging on the impeller radially outwardly into the side wall and up the side wall thereby creating a standing vortex,

wherein;

the manifold is connected to the water delivery system to receive water and deliver water to the water inlet of the tank when the manifold and tank are coupled,

the manifold is connected to the air delivery system to receive air and deliver air to the air inlet of the tank when the manifold and tank are coupled,

the manifold is connected to the nutrient delivery system to receive nutrients and deliver nutrients to the nutrient inlet of the tank when the manifold and tank are coupled,

the impeller is rotatable about the axis to creating the standing vortex thereby raising fluid in the tank up the side wall to heights within the tank which increase with increased speed of rotation of the impeller,

the impeller is provided internally within the tank and is coupled through the bottom of the tank with a mechanism to rotate the impeller via a second quick connect and disconnect arrangement,

wherein the steps of coupling each tank to the remainder of the apparatus and uncoupling of each tank from the remainder of the apparatus consists of use of the first and second quick connect and disconnect arrangements.

13. A process as claimed in claim 12 wherein the tank defines an enclosed compartment without any openings into the compartment other than the open top of the tank.

14. A process as claimed in claim 12 wherein the tank side wall and bottom do not have any openings therethrough such that the only opening into the tank is the open top of the tank forming the tank outlet.

15. A process as claimed in claim 14 wherein the agitation mechanism includes a motor external of the tank magnetically coupled to the impeller through the bottom of the tank.

16. A process as claimed in claim 12 wherein the apparatus further comprises an electric motor external of the tank coupled to the impeller to rotate the impeller and a power supply to the motor;

the motor is at the bottom of the tank, the motor is coupled to the impeller by a shaft extending in fluid sealed relation through a shaft opening in the bottom of the tank to the impeller within the tank,

the tank side wall does not have any openings therethrough with the only opening into the tank being the shaft opening in the bottom and the open top of the tank forming the tank outlet,

the second quick connect and disconnect arrangement being external of the tank and being either between the motor and the power supply or between the motor and the shaft.

17. A process as claimed in claim 16 wherein the motor and impeller are coupled to the tank to be removable and replaceable with the tank,

the agitation mechanism including the power supply to the motor being removably coupled to the motor by the second quick connect and disconnect arrangement.

18. A method of dispensing fluid from a container, the container having a base, side walls extending upwardly from the base and an exit opening at a height above the base,

the method characterized in comprising:

providing fluid in the container at a height below the exit opening,

providing an impeller in the container rotatable about an axis to discharge fluid impinging on the impeller,

rotating the impeller for a period of time at speeds which create and maintain a standing vortex in the container with a height of the standing vortex being below a height of the exit opening, and

to dispense fluid from the container, increasing the speed of rotation of the impeller to increase the height of the standing vortex to a height of the exit opening such that fluid above the exit opening exits the container via the exit opening.

19. A method as claimed in claim 18 wherein the impeller creates a vortex by directing fluid radially outwardly into the side walls and up the side walls.

20. A method as claimed in claim 19 wherein the side wall is generally circular in cross-section normal the axis and disposed co-axially about the axis, the axis is generally vertical, and

the side wall is disposed at a radius about the axis, the radius of the wall increasing as the wall extends upwardly.