Protocols for making hepatocytes from embryonic stem cells
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Abstract
This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.
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Citations
31 Claims
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1-22. -22. (canceled)
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23. A method of screening a compound for an effect on a hepatocyte lineage cell in cell culture, comprising:
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a. contacting the cell culture with the compound; and b. observing a change in the hepatocyte lineage cells in the cell culture, wherein the cell culture comprises hepatocyte lineage cells that are the progeny of a non-malignant in vitro cell culture comprising cells which express SSEA3, SSEA4, Tra-1-60 and Tra-1-81. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31)
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Specification