CELL SEPARATION USING MICROCHANNEL HAVING PATTERNED POSTS
First Claim
1. A microflow apparatus comprising:
- a body having a randomized flow path which comprises an inlet means, an outlet means, and a microchannel arrangement extending between said inlet and outlet means,wherein said microchannel arrangement includes a plurality of transverse separator posts being integral with a base surface of said microchannel and projecting therefrom, wherein said posts are arranged in a pattern capable of providing said randomized flow path, and wherein said inlet means comprises an inlet passageway that is aligned at an angle of between about 30°
to about 60°
to said base surface.
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Accused Products
Abstract
A micro now device (11, 71) for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts (23, 81). The posts are spaced across the width of an expanded collection chamber region (17, 75) in the flow path, extending between the upper and lower surfaces thereof: they have rectilinear surfaces, being curved in cross-sections, e.g. circular or tear-drop shaped, and are randomly arranged so as to disrupt streamlined flow. The device is oriented so that its lower surface is aligned at about 45° to the horizontal. Sequestering agents, such as Abs, which are attached to surfaces of the collection region via a hydrophilic coating, preferably a permeable hydrogel containing isocyanate moieties, are highly effective in capturing cells or other targeted biomolecules while the remainder of the liquid sample exits horizontally.
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Citations
21 Claims
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1. A microflow apparatus comprising:
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a body having a randomized flow path which comprises an inlet means, an outlet means, and a microchannel arrangement extending between said inlet and outlet means, wherein said microchannel arrangement includes a plurality of transverse separator posts being integral with a base surface of said microchannel and projecting therefrom, wherein said posts are arranged in a pattern capable of providing said randomized flow path, and wherein said inlet means comprises an inlet passageway that is aligned at an angle of between about 30°
to about 60°
to said base surface. - View Dependent Claims (2, 3, 4, 5, 6, 7, 16, 17, 18)
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8. A microflow apparatus comprising:
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a body having a randomized flow path which comprises an inlet means, an outlet means, and a microchannel arrangement extending between said inlet and outlet means, wherein said microchannel arrangement includes a plurality of transverse separator posts being integral with a base surface of said microchannel and projecting therefrom, wherein said posts are arranged in a pattern capable of providing said randomized flow path, and wherein the body has a flat surface and the microchannel arrangement is located in a cavity of the body below the flat surface. - View Dependent Claims (9, 10, 11, 12, 13, 14, 15, 19, 20, 21)
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Specification