METHOD FOR DETERMINING THE QUALITY OF A BIOLOGICAL SAMPLE
First Claim
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1. A method for identifying a biological marker for the quality of a biological sample comprising the steps;
- a) detecting the presence and amount of degradation products of proteins and peptides in a test sample at one or more time points within 10 minutes post sampling, andb) identifying a degradation product which is formed in a time dependent manner within 10 minutes post sampling as a biological marker for the quality of a biological sample.
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Abstract
The present invention relates to a method of determining the quality of a biological sample. A method according to the invention may be used to determine if a biological sample is suitable to use in a further biological assay demanding samples of good quality to render an accurate result. The method comprises detecting the presence of protein fragments in the biological sample by using appropriate means. The invention also relates to a kit for determining the quality of a biological sample.
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36 Claims
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1. A method for identifying a biological marker for the quality of a biological sample comprising the steps;
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a) detecting the presence and amount of degradation products of proteins and peptides in a test sample at one or more time points within 10 minutes post sampling, and b) identifying a degradation product which is formed in a time dependent manner within 10 minutes post sampling as a biological marker for the quality of a biological sample. - View Dependent Claims (2, 3)
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- 4. A method for determining the quality of a biological sample, wherein said quality is determined by detecting the presence and/or amount of a degradation product of a protein or a peptide, wherein said degradation product has been identified to be formed in a time dependent manner within 10 minutes post sampling in a comparable test sample.
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19. The peptide SEQ ID NO:
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- 20. An antibody directed to the peptide SEQ ID NO:
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22. A kit for determining the quality of a biological sample, comprising an antibody to stathmin, optionally in combination with suitable reagents, for detecting the presence of stathmin and/or a fragment of stathmin in said sample.
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24. A biological marker for determining the quality of a biological sample, characterized by:
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a) being formed post sampling as a degradation product of a protein or a peptide present in said biological sample, and b) being formed in a time dependent manner within 10 minutes post sampling in a comparable untreated test sample at 25°
C. - View Dependent Claims (25, 26, 27, 28, 29, 30, 31)
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32. A method for determining the quality of a biological sample, wherein said quality is determined by detecting the total amount of degradation products of proteins and peptides in said sample, wherein
a) said degradation products are peptide fragments with a molecular weight less than 10 kDa, and b) the quality of said biological sample is determined by comparing the total amount of peptide fragments present in the biological sample with the standard amount of peptide fragments and endogenous peptides present in comparable biological samples of high quality.
Specification