Manipulation of Microparticles In Microfluidic Systems

• US 20090137413A1
• Filed: 10/30/2007
• Published: 05/28/2009
• Est. Priority Date: 02/23/1999
• Status: Abandoned Application

• Current Assignee: Caliper Life Sciences Incorporated (Revvity, Inc.)
• Original Assignee: Caliper Life Sciences Incorporated (Revvity, Inc.)
• Inventors: Chow, Andrea W., Parce, J. Wallace, Knapp, Michael R., Kopf-Sill, Anne R., Mehta, Tammy Burd, Gallagher, Steve, Bousse, Luc J., Nikiforov, Theo T.
• Application Number:
  US11/928,808
• Publication Number:
  US 20090137413A1
• Time in Patent Office: Days
• US Class Current: 506/9
• CPC Class Codes: B01F 33/30 Micromixers
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00459 Beads
• CPC Class Codes: B01J 2219/00468 by manipulation of individual beads
• CPC Class Codes: B01L 2200/0668 Trapping microscopic beads
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 2400/043 magnetic forces
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: C07H 21/02 with ribosyl as saccharide radical
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: C12Q 2525/186 incorporating a non-extendable or blocking moiety
• CPC Class Codes: C12Q 2527/125 Specific component of sample, medium or buffer
• CPC Class Codes: C12Q 2535/101 Sanger sequencing method, i.e. oligonucleotide sequencing using primer elongation and dideoxynucleotides as chain terminators
• CPC Class Codes: C12Q 2563/173 staining/intercalating agent, e.g. ethidium bromide
• CPC Class Codes: C12Q 2565/629 : being a microfluidic device
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: G01N 2035/00574 : Means for distributing beads
• CPC Class Codes: G01N 27/44756 : Apparatus specially adapted therefor
• CPC Class Codes: G01N 35/0098 : involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: Y10T 436/143333 : Saccharide [e.g., DNA, etc.]

Abstract

Arrays of flowable or fixed particle sets are used in microfluidic systems for performing assays and modifying hydrodynamic flow. Also provided are assays utilizing flowable or fixed particle sets within a microfluidic system, as well as kits, apparatus and integrated systems comprising arrays and array members.

Claims

1. A method of carrying out a chemical reaction in a microfluidic device, the method comprising:

providing a microfluidic device comprising a body structure with a microscale cavity disposed therein, wherein the microscale cavity comprises a first microchannel comprising at least one reagent flow region and at least one particle capture region, and wherein the at least one particle capture region has an increased depth relative to the at least one reagent flow region;

flowing a set of particles through the microscale cavity to the particle capture region where the set of particles is retained in a fixed position; and

flowing one or more liquid reagents over the retained set of particles so that a chemical reaction occurs within the particle retention region.

2. The method of claim 1, wherein the chemical reaction comprises a reaction between at least one of the one or more liquid reagents and the particles.

3. The method of claim 1, wherein the chemical reaction comprises a reaction between at least one of the one or more liquid reagents and reagents coupled to the particles in the set of particles.

4. The method of claim 3, wherein the chemical reaction comprises nucleic acid hybridization.

5. The method of claim 3, wherein the chemical reaction comprises DNA amplification.